Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae

Sakakibara, Mako; Hasegawa, Tomoka; Haraguchi-Kitakamae, Mai; Shi, Yan; Li, Weisong; Cui, Jiaxin; Liu, Xuanyu; Yamamoto, Tomomaya; Hongo, Hiromi; Amizuka, Norio; Sato, Yoshiaki; Kikuchi, Masanori

Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae

Sakakibara, Mako; Hasegawa, Tomoka; Haraguchi-Kitakamae, Mai et al.

Journal of oral biosciences, 2025, 67(1)

Permalink : https://hdl.handle.net/2115/97415

このアイテムのアクセス数:50件（2026-07-15 01:12 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/97415
タイトル	Histochemical analysis of osteoclast and osteoblast distributions on hydroxyapatite/collagen bone-like nanocomposite embedded in rat tibiae
著者	Sakakibara, Mako ; Hasegawa, Tomoka ; Haraguchi-Kitakamae, Mai ; Shi, Yan ; Li, Weisong ; Cui, Jiaxin ; Liu, Xuanyu ; Yamamoto, Tomomaya ; Hongo, Hiromi ; Amizuka, Norio ; Sato, Yoshiaki ; Kikuchi, Masanori
著者名 Sakakibara, Mako
e-Rad_Researcher 50739349 著者名 Hasegawa, Tomoka
著者名 Haraguchi-Kitakamae, Mai
著者名 Shi, Yan
著者名 Li, Weisong
著者名 Cui, Jiaxin
著者名 Liu, Xuanyu
e-Rad_Researcher 50928299 著者名 Yamamoto, Tomomaya
e-Rad_Researcher 00778970 著者名 Hongo, Hiromi
e-Rad_Researcher 30242431 著者名 Amizuka, Norio
e-Rad_Researcher 00250465 著者名 Sato, Yoshiaki
e-Rad_Researcher 00354267 著者名 Kikuchi, Masanori
言語	英語
キーワード	HAp/Col
	Bone regeneration
	Osteoblast
	Osteoclast
	Histochemistry
発行日	2025-03
出版者	Elsevier
収録物名	Journal of oral biosciences
巻(号)	67(1)
抄録	Objectives: Hydroxyapatite (HAp)/collagen (Col) cylinders with laminated collagen layers were implanted into the tibial diaphysis of rats and examined histochemically to clarify how the orientation of HAp and Col bone-like nanocomposite fibers in HAp/Col blocks affects bone resorption and formation. Methods: HAp/Col fibers were synthesized and compressed into cylindrical blocks to mimic bone nanostructures. These were implanted into the cortical bone cavities of 10-week-old male Wistar rats with fiber bundles parallel to the tibial surface. The implants were histologically analyzed at 3, 5, 7, 14, and 28 days after implantation. Results: TRAP-positive osteoclasts appeared after 3-5 days in the lateral region of the graft, where the fiber ends were exposed, but not in the bottom region, where the HAp/Col fibers were parallel to the surface. Osteoclasts were observed in both regions by day 14. PHOSPHO1-positive osteoblasts were first detected on day 5, appearing slightly away from the cylinder laterally but directly on the bottom surface. A few osteoblasts contacted the block laterally, whereas many were observed on the new bone tissue at the bottom, between days 7 and 14. Bone formation was induced earlier in the bottom region, whereas lateral resorption was dominant. This suggested the uncoupling of bone resorption and formation in the early postimplantation stages. However, bone remodeling shifted to coupling between osteoclasts and osteoblasts throughout the cylinder by day 28. Conclusion: The orientation of HAp/Col fibers in HAp/Col graft materials substantially affected the preferential induction of bone resorption or formation during the early stages of bone regeneration.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2025. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1349-0079
EISSN	1880-3865
NCID	AA11896386
関連情報	PMID 39805401
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.job.2025.100612