open access

https://hdl.handle.net/2115/97475

Alkylglycerol enhances myogenesis and regulates ether-phospholipid metabolism in C2C12 myoblasts

英語

C2C12

2025-03-25

Elsevier

Biochemical and biophysical research communications

754

151532

1-O-Alkylglycerol (AKG), a lipid characteristic of marine organisms, possesses an ether-linked alkyl chain on its glycerol backbone. AKG exhibits various biological activities, including anti-cancer effects, promoting sperm motility, and stimulating immune response. Metabolically, AKG is converted into alkyl- and alkenylphospholipids (PLs), which are key components of the cell membrane and play essential roles in maintaining membrane homeostasis and cellular functions. However, the influence of AKG on myogenesis and ether-type PL metabolism in muscle cells remains unknown. This study evaluated the effects of AKG on myogenic differentiation and ether-PL metabolism in mouse C2C12 myoblasts. During differentiation, cells were treated with 10-20 mu M 1-O-octadecyl-glycerol (batyl alcohol) and 1-O-hexadecyl-glycerol (chimyl alcohol). By day 7 of differentiation, myotube size had increased in cells treated with AKGs. Comparative tests using compounds with similar or partial structures, including monoacylglycerol and alkenylglycerol, demonstrated that this activity was linked to the structural features of AKG. Conversely, myotube growth was insufficient after treatment with 1-O-dodecylglycerol, which contains a shorter alkyl chain. Additionally, batyl alcohol treatment elevated the levels of etherphosphatidylcholine (PC) molecular species, including e-PC38:4 and e-PC38:5, those are presumed to bind polyunsaturated fatty acids. Chimyl alcohol treatment also increased ether-PC species, including e-PC36:4 and ePC36:5 while monoacylglycerol did not alter ether-PC levels. These findings suggest that AKG plays a crucial role in membrane dynamics during myogenesis through metabolic conversion to ether-PLs, providing novel insights into muscle homeostasis to contribute to developing nutritional strategies and preventing and treating muscle diseases.

学術雑誌論文

Accepted Manuscript

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

© 2025. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/

0006-291X

1090-2104

AA00564395