open access

https://hdl.handle.net/2115/97567

Extracellular matrix modulating effects of amnion-derived mesenchymal stem cells on aging skin wounds in α-Klotho knockout mice

英語

Wound healing

2025-04-14

John Wiley & Sons

Geriatrics & gerontology international

25(5)

701-708

Aim Wounds in the elderly are frequently recalcitrant and chronic as a result of the effects of skin aging and associated complications. The objective of this study is to utilize an alpha-Klotho knockout (KO) mice wound model to assess the capacity of amnion-derived mesenchymal stem cells (AMSCs) to facilitate wound healing in aging skin.Methods AMSCs were applied topically to the wound after extraction and gelatinization of the conditioned medium (CM). Animal experiments were performed with two distinct mouse strains: alpha-Klotho KO mice and wild-type mice. Full-thickness skin defect models with a diameter of 8 mm were created by incising the skin on the left and right sides of the dorsum. On day 8 after wound creation, the mice were sacrificed, and wound tissue was collected for analysis through histological and immunohistochemical evaluations, as well as through quantitative polymerase chain reaction.Results The topical application of CM gel to wounds of alpha-Klotho KO mice demonstrated that wound healing was significantly higher than that observed in control, reaching the wound closure rate of wild-type mice on day 8. Additionally, gene expression analysis of wound tissue indicated that AMSC-CM may regulate extracellular matrix formation and fibrosis. Moreover, histological analysis indicated that AMSC-CM may facilitate wound contraction of aging skin wounds of alpha-Klotho KO mice by inducing myofibroblast differentiation and promoting granulation and collagen formation, which are the primary components of the extracellular matrix.Conclusions AMSC-CM may facilitate wound healing in aging skin of alpha-Klotho KO mice by regulating the extracellular matrix. Geriatr Gerontol Int 2025; 25: 701-708.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Sasaki Y, Ohnishi S, Takahashi H, et al. Extracellular matrix modulating effects of amnion-derived mesenchymal stem cells on aging skin wounds in α-Klotho knockout mice. Geriatr. Gerontol. Int. 2025; 25: 701–708. https://doi.org/10.1111/ggi.70043, which has been published in final form at https://doi.org/10.1111/ggi.70043. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

1444-1586

1447-0594