Identification of novel factors involved in polarized cell expansion and development in the moss, Physcomitrium patens

Singh, Prerna

doi:https://doi.org/10.14943/doctoral.k16288

Identification of novel factors involved in polarized cell expansion and development in the moss, Physcomitrium patens

Singh, Prerna

2025

Permalink : https://doi.org/10.14943/doctoral.k16288

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アクセス権	open access
DOI	https://doi.org/10.14943/doctoral.k16288
URI	https://hdl.handle.net/2115/97696
タイトル	Identification of novel factors involved in polarized cell expansion and development in the moss, Physcomitrium patens
タイトル	コケ植物Physcomitrium patensにおける極性細胞の伸長と発生に関与する新規因子の同定
著者	著者名 Singh, Prerna
言語	英語
発行日	2025-03-25
出版者	Hokkaido University
ページ数	65p
抄録	Tip growth is vital for plant growth and development, yet the regulatory mechanisms governing this process remain incompletely understood. In this study, I identified Reagent F4, a novel small molecule that disrupts tip growth and polarized cell expansion in the moss, Physcomitrium patens protonemata. Through unbiased chemical screening, I found that Reagent F4 induces abnormal protonemal morphology, characterized by reduced cell elongation and stunted cell expansion. My analyses revealed that F4 treatment triggers actin depolymerization and disrupts apical actin foci, which are critical for initiating and maintaining tip growth. Additionally, both acute and prolonged F4 exposure led to mislocalization of ROP GTPase, a key regulator of cell polarity. Transcriptomic analyses of F4 treated protonemata show significant downregulation of genes involved in lipid asymmetry, a process essential for polarized growth. These findings establish Reagent F4 as a valuable tool to investigate the molecular mechanisms governing tip growth in P. patens and highlight the potential role of lipid asymmetry in coordinating cytoskeletal organization and membrane polarity.
学位授与機関	識別子 10101 機関名北海道大学 Hokkaido University
学位授与年月日	2025-03-25
学位授与番号	甲第16288号
学位名	博士(生命科学)
学位の審査委員	(主査) 教授藤田知道, 教授千葉由佳子, 准教授楢本悟史, 准教授佐藤長緒
学位審査の研究科等	生命科学院（生命科学専攻）
資源タイプ	博士論文
出版タイプ	Version of Record
関連情報 (isReferencedBy)	HDL https://hdl.handle.net/2115/95521 Title Identification of novel factors involved in polarized cell expansion and development in the moss, Physcomitrium patens [an abstract of dissertation and a summary of dissertation review]
関連情報 (isReferencedBy)	HDL https://hdl.handle.net/2115/95522 Title Identification of novel factors involved in polarized cell expansion and development in the moss, Physcomitrium patens [an abstract of entire text]