Structural and Stability Analysis of GRP Family Allergens Pru p 7 and Cry j 7, Which Cause Pollen and Food Allergy Syndrome

鄭, 靖康

doi:https://doi.org/10.14943/doctoral.k16296

Structural and Stability Analysis of GRP Family Allergens Pru p 7 and Cry j 7, Which Cause Pollen and Food Allergy Syndrome

鄭, 靖康

2025

Permalink : https://doi.org/10.14943/doctoral.k16296

このアイテムのアクセス数:59件（2026-10-17 16:13 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Jingkang_Zheng	pdf	6.57 MB	126

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
DOI	https://doi.org/10.14943/doctoral.k16296
URI	https://hdl.handle.net/2115/97704
タイトル	Structural and Stability Analysis of GRP Family Allergens Pru p 7 and Cry j 7, Which Cause Pollen and Food Allergy Syndrome
タイトル	花粉食物アレルギー症候群の原因となるGRPファミリーアレルゲンPru p 7およびCry j 7の構造および安定性の解析
著者	著者名鄭, 靖康
言語	英語
発行日	2025-03-25
出版者	Hokkaido University
ページ数	88p
抄録	Cry j 7 is a 7 kDa cysteine-rich gibberellin regulatory protein (GRP) with six　disulfide bonds. It was isolated from Japanese cedar as the pollen allergen in this study.　It exhibits cross-reactivity with food allergens such as Pru p 7 from peach and causes pollen-food allergy syndrome (PFAS). In this work, recombinant Cry j 7 and Pru p 7 were successfully overexpressed using Pichia pastoris in a high-cell-density fermentation culture, and pure proteins were purified by reverse phase-HPLC. The characterization of Cry j 7 and Pru p 7 were performed by MS, CD, and 1 H-NMR experiments, to confirm the correct native conformation of Cry j 7 as well as Pru p 7. When compared to the results showed that Cry j 7 exhibits excellent stability in disulfide linkages and preserves its original structure up to 90 ˚C in various pH buffers in comparison to Pru p 7. Notably, NMR analyses indicated the greater mobility in the α-helix and loop regions of S38-C47 in Pru p 7 compared to those of Cry j 7. Furthermore, our results showed that the sensitivity of Cry j 7 to enzyme digestion differed from that of Pru p 7: the Cry j 7 was more susceptible to proteolysis, while Pru p 7 displayed better resistance in the gastrointestinal tract. These variations in structural stability and sensitivity to proteolysis provide valuable insights into the allergenicity within the GRP family.
学位授与機関	識別子 10101 機関名北海道大学 Hokkaido University
学位授与年月日	2025-03-25
学位授与番号	甲第16296号
学位名	博士(ソフトマター科学)
学位の審査委員	(主査) 教授相沢智康, 教授比能洋, 教授尾瀬農之
学位審査の研究科等	生命科学院（ソフトマター専攻）
資源タイプ	博士論文
出版タイプ	Version of Record
関連情報 (isReferencedBy)	HDL https://hdl.handle.net/2115/95465 Title Structural and Stability Analysis of GRP Family Allergens Pru p 7 and Cry j 7, Which Cause Pollen and Food Allergy Syndrome [an abstract of dissertation and a summary of dissertation review]