Histochemical assessment of the anabolic effects of abaloparatide on the femoral metaphyses of ovariectomized mice

Liu, Xuanyu; Hasegawa, Tomoka; Sakakibara, Mako; Yamamoto, Tomomaya; Haraguchi-Kitakamae, Mai; Ishizu, Hotaka; Shi, Yan; Cui, Jiaxin; Li, Weisong; Haoyu, Wang; Hongo, Hiromi; Shimizu, Tomohiro; Ohiro, Yoichi; Amizuka, Norio

Histochemical assessment of the anabolic effects of abaloparatide on the femoral metaphyses of ovariectomized mice

Liu, Xuanyu; Hasegawa, Tomoka; Sakakibara, Mako et al.

Journal of oral biosciences, 2025, 67(2), 100663

Permalink : https://hdl.handle.net/2115/97810

このアイテムのアクセス数:80件（2026-10-06 16:31 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/97810
タイトル	Histochemical assessment of the anabolic effects of abaloparatide on the femoral metaphyses of ovariectomized mice
著者	Liu, Xuanyu ; Hasegawa, Tomoka ; Sakakibara, Mako ; Yamamoto, Tomomaya ; Haraguchi-Kitakamae, Mai ; Ishizu, Hotaka ; Shi, Yan ; Cui, Jiaxin ; Li, Weisong ; Haoyu, Wang ; Hongo, Hiromi ; Shimizu, Tomohiro ; Ohiro, Yoichi ; Amizuka, Norio
著者名 Liu, Xuanyu
e-Rad_Researcher 50739349 著者名 Hasegawa, Tomoka
著者名 Sakakibara, Mako
e-Rad_Researcher 50928299 著者名 Yamamoto, Tomomaya
著者名 Haraguchi-Kitakamae, Mai
著者名 Ishizu, Hotaka
著者名 Shi, Yan
著者名 Cui, Jiaxin
著者名 Li, Weisong
著者名 Haoyu, Wang
e-Rad_Researcher 00778970 著者名 Hongo, Hiromi
e-Rad_Researcher 60784246 著者名 Shimizu, Tomohiro
e-Rad_Researcher 40301915 著者名 Ohiro, Yoichi
e-Rad_Researcher 30242431 著者名 Amizuka, Norio
言語	英語
キーワード	Abaloparatide
	Bone formation
	Bone histomorphometry
	Osteoblast
	Osteoclast
発行日	2025-04-18
出版者	Elsevier
収録物名	Journal of oral biosciences
巻(号)	67(2)
ページ	100663
抄録	Objective: To clarify the mechanism of bone anabolism induced by the parathyroid hormone-related peptide analog abaloparatide, we histochemically examined the femora of ovariectomized mice treated with abaloparatide. Methods: Twelve-week-old female C57BL/6J mice underwent ovariectomies (OVX), and were then administered either abaloparatide (30 mu g/kg/day: OVX + ABL group) or vehicle (OVX group) via daily intraperitoneal injection. Femora were harvested at 0, 2, 4, and 6 weeks post-administration and subjected to micro-CT imaging, TRAP, cathepsin K, ALP, and PHOSPHO1 staining, along with calcein labeling. Results: In the OVX group, trabecular number and bone volume gradually decreased over time, whereas the OVX + ABL group maintained these values to 6 weeks after OVX. The numbers of TRAP-positive/cathepsin K-reactive osteoclasts per bone surface area were similar between the OVX and OVX + ABL group, except for a temporary increase at 4 weeks in the OVX group. In the OVX group, the areas of ALP-positive osteoblastic cells and PHOSPHO1-reactive mature osteoblasts decreased, whereas in the OVX + ABL group, ALP-positive osteoblastic cells surrounded the trabeculae, and long lines of PHOSPHO1-reactive mature osteoblasts expanded to the terminal region of the trabeculae. In addition, long continuous calcein labeling was seen on slightly convex new bone, indicating modeling-based bone formation in the OVX + ABL group. The bone formation rate/bone surface ratio and the total length of modeling-based bone formation sites were higher in the OVX + ABL group than in the OVX group. Conclusion: Abaloparatide suppresses bone loss following ovariectomy by promoting both remodeling-based and modeling-based bone formation.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	©2025. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1349-0079
EISSN	1880-3865
関連情報	PMID 40254128
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.job.2025.100663