open access

https://hdl.handle.net/2115/97862

Advanced fixation techniques for high-sensitivity molecular imaging : effectiveness of glyoxal fixation for immunostaining

英語

Glyoxal

2025-05-26

Springer

Anatomical science international

100(4)

562-567

Chemical fixation is essential in immunohistochemistry for detecting molecular localization. The gold-standard fixative for immunohistochemistry is 4% paraformaldehyde (PFA); however, its properties-such as tissue shrinkage and protein cross-linking through methylene bridges-often restrict antibody access, posing challenges to achieving specific binding reactions. This is particularly true for receptors and ion channels condensed in the synaptic cleft, postsynaptic density, or trigger zone of action potentials. To overcome this problem, several laboratories have attempted antigen-exposure techniques. Recently, we demonstrated that fixation by glyoxal, a dialdehyde with two carbon atoms, enables specific detection of molecular groups that are difficult to be detected in PFA-fixed tissues. Here, we summarize the advantages and precautions in the use of glyoxal fixative.

学術雑誌論文

Accepted Manuscript

This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s12565-025-00848-z

1447-6959

1447-073X