Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal

Izumi, Hikari; Uji, Toshiki; Matsumoto, Kojiro; Nagaosa, Kaz; Fukuda, Satoru

Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal

Izumi, Hikari; Uji, Toshiki; Matsumoto, Kojiro et al.

Marine Biotechnology, 2025, 27, 123

Permalink : https://hdl.handle.net/2115/98046

このアイテムのアクセス数:11件（2026-09-06 09:46 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/98046
タイトル	Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal
著者	Izumi, Hikari ; Uji, Toshiki ; Matsumoto, Kojiro ; Nagaosa, Kaz ; Fukuda, Satoru
著者名 Izumi, Hikari
e-Rad_Researcher 00760597 著者名 Uji, Toshiki
著者名 Matsumoto, Kojiro
著者名 Nagaosa, Kaz
e-Rad_Researcher 20520951 著者名 Fukuda, Satoru
言語	英語
キーワード	Gene delivery
	Electroporation
	Red macroalgae
	Pyropia yezoensis
発行日	2025
出版者	Springer
収録物名	Marine Biotechnology
巻(号)	27
ページ	123
抄録	This study aimed to assess the potential of a gene delivery technique in the red macroalga Pyropia yezoensis (Rhodophyta) using electroporation without removing the cell wall. An antibiotic resistance gene was introduced into P. yezoensis tissues containing cells with intact cell walls through electroporation, followed by selection with the corresponding antibiotic. No germlings survived in the non-electroporated control tissue fragments under antibiotic selection. In contrast, some germlings were observed to survive in the electroporated group. Furthermore, the presence of antibiotic resistance genes was confirmed in the genomic DNA of several antibiotic-resistant germlings. Although reporter genes such as beta-glucuronidase (GUS) and green fluorescent protein (GFP) were also introduced as supplementary markers, their expression was not detectable under the tested conditions. These findings provide evidence supporting the successful introduction of antibiotic resistance genes into P. yezoensis cells via electroporation. This study offers a preliminary assessment of a gene delivery strategy in P. yezoensis that bypasses cell wall removal, presenting a straightforward method for introducing foreign genes into Pyropia. To the best of our knowledge, this is the first report to demonstrate successful gene transfer via electroporation in a macroalga without cell wall removal. These results provide valuable insights for the development of genetic transformation systems in red macroalgae.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10126-025-10506-0
PISSN	1436-2228
EISSN	1436-2236
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10126-025-10506-0