Important constituents of heavy water-containing solution for cold storage and subsequent reperfusion on an isolated perfused rat liver

Fukai, Moto; Sakamoto, Sodai; Shibata, Kengo; Ishikawa, Takahisa; Kawamura, Norio; Fujiyoshi, Masato; Fujiyoshi, Sunao; Nakamura, Kosei; Bochimoto, Hiroki; Shimada, Shingo; Shimamura, Tsuyoshi; Taketomi, Akinobu

Important constituents of heavy water-containing solution for cold storage and subsequent reperfusion on an isolated perfused rat liver

Fukai, Moto; Sakamoto, Sodai; Shibata, Kengo et al.

Transplantation Proceedings, 2024, 56(1), 223-227

Permalink : https://hdl.handle.net/2115/98925

このアイテムのアクセス数:31件（2026-07-15 01:16 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/98925
タイトル	Important constituents of heavy water-containing solution for cold storage and subsequent reperfusion on an isolated perfused rat liver
著者	Fukai, Moto ; Sakamoto, Sodai ; Shibata, Kengo ; Ishikawa, Takahisa ; Kawamura, Norio ; Fujiyoshi, Masato ; Fujiyoshi, Sunao ; Nakamura, Kosei ; Bochimoto, Hiroki ; Shimada, Shingo ; Shimamura, Tsuyoshi ; Taketomi, Akinobu
e-Rad_Researcher 60374344 著者名 Fukai, Moto
著者名 Sakamoto, Sodai
e-Rad_Researcher 90962853 著者名 Shibata, Kengo
著者名 Ishikawa, Takahisa
e-Rad_Researcher 20746953 著者名 Kawamura, Norio
e-Rad_Researcher 90844720 著者名 Fujiyoshi, Masato
e-Rad_Researcher 00892530 著者名 Fujiyoshi, Sunao
著者名 Nakamura, Kosei
著者名 Bochimoto, Hiroki
著者名 Shimada, Shingo
e-Rad_Researcher 00333617 著者名 Shimamura, Tsuyoshi
e-Rad_Researcher 70363364 著者名 Taketomi, Akinobu
言語	英語
発行日	2024-01-09
出版者	Elsevier
収録物名	Transplantation Proceedings
巻(号)	56(1)
ページ	223-227
抄録	The University of Wisconsin (UW) solution is the most effective preservation solution currently used; however, to safely use expanded-criteria donor grafts, a new cold storage solution that alleviates graft injury more effectively is required. We prepared a heavy water (D2O)-containing buffer, Dsol, and observed strong protective effects during extended cold storage of rat hearts and livers. In the current study, we modified Dsol (mDsol) and tested its efficacy. The aim of the present study was to determine whether mDsol could protect the rat liver more effectively than the UW solution and to clarify the roles of D2O and deferoxamine (DFX). Rat livers were subjected to cold storage for 48 hours in test solutions: UW, mDsol, mDsol without D2O or DFX (mDsol-D2O[-], mDsol-DFX[-]), and subsequently reperfused on an isolated perfused rat liver for 90 minutes at 37°C. In the UW group, the liver was dehydrated during cold storage and rapidly expanded during reperfusion. Accordingly, the cumulative weight change was the highest in the UW group, together with augmented portal veinous resistance and ALT leakage and decreased oxygen consumption rate and bile production. These changes were significantly suppressed in the mDsol-treated group. In the mDsol-D2O(-) and mDsol-DFX(-) groups offered partial protection. In conclusion, mDsol appeared to be superior to the UW solution for simple cold storage of the rat liver, presumably due to improved microcirculation in the early phase of reperfusion. Both heavy water and deferoxamine are essential for alleviating seamless organ swelling that occurs during cold storage and subsequent reperfusion.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0041-1345
EISSN	1873-2623
関連情報	PMID 38199859
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.transproceed.2023.10.005