open access

https://hdl.handle.net/2115/998

Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars

英語

pollen fertility

2005

Blackwell Science Ltd

New Phytologist

166(2)

455-464

The definitive version is available at www.blackwell-synergy.com

A successful androgenesis in amphidiploid Festulolium (Lolium perenne L. x Festuca pratensis Huds., 2n=4x=28) was obtained using PG-96 medium for embryo/callus induction. The green plant regeneration varied, and was 46 %, 35 % and 17 % for Bx350, Bx351 and Prior, respectively and over 800 green plants have been obtained. Androgenic progeny showed a large variation in freezing tolerance, 7 % of 292 progeny exceeding that of freezing hardy F. pratensis despite containing chromosomes of L. perenne, a more freezing-sensitive species. More than 60% of flowering 175 progeny produced dehiscent anthers with pollen stainability ranging from 5% to 85%. Androgenic plants contained 14 or 28 chromosomes. There were 188 (56 %), 204 (77 %) and 114 dihaploids (81 %) from Bx350, Bx351 and Prior, respectively. However, the nuclear DNA content varied significantly even between plants with the same chromosome number. Variation in DNA content reflected the genetic variation inherent in androgenic populations. High levels of chromosome pairing and recombination were observed due to close homology between genomes of L. perenne and F. pratensis.

学術雑誌論文

Accepted Manuscript

0028-646X

1469-8137