Identification of cultivars and accessions of Lolium, Festuca and Festulolium hybrids through the detection of simple sequence repeat (SSR) polymorphism

Momotaz, Aliya; Forster, John W.; Yamada, Toshihiko

Identification of cultivars and accessions of Lolium, Festuca and Festulolium hybrids through the detection of simple sequence repeat (SSR) polymorphism

Momotaz, Aliya; Forster, John W.; Yamada, Toshihiko

Plant Breeding, 2003, 123(4), 370-376

Permalink : https://hdl.handle.net/2115/999

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アクセス権	open access
URI	https://hdl.handle.net/2115/999
タイトル	Identification of cultivars and accessions of Lolium, Festuca and Festulolium hybrids through the detection of simple sequence repeat (SSR) polymorphism
著者	Momotaz, Aliya ; Forster, John W. ; Yamada, Toshihiko
著者名 Momotaz, Aliya
著者名 Forster, John W.
e-Rad_Researcher 70343952 著者名 Yamada, Toshihiko 山田, 敏彦
言語	英語
キーワード	Festuca arundinacea
	F. pratensis
	Festulolium
	Lolium multiflorum
	L. perenne
	Lolium/Festuca complex
	genetic diversity
	SSR marker
発行日	2003
出版者	Blackwell Verlag
収録物名	Plant Breeding
巻(号)	123(4)
ページ	370-376
記述	The definitive version is available at www.blackwell-synergy.com
抄録	Molecular diversity and genetic affinity in the Lolium/Festuca grass complex have been assessed using simple sequence repeat (SSR) marker technology. The genotypic set was derived from three accessions of Lolium perenne, two cultivars of Lolium multiflorum, two cultivars of Festuca pratensis, two cultivars of Festuca arunidinacea and ten accessions from different intergeneric hybrid (Festulolium) combinations. The majority of the genomic DNA-derived SSR primer pairs from perennial ryegrass (LPSSR) and Italian ryegrass (LMSSR) produced clear, simple and distinctive amplification products from the majority of the genotypes. The efficiency of cross-specific amplification for LPSSR markers varied from 38% in F. pratensis to 93% in two cultivars of Festulolium and for LMSSR markers from 57% in F. pratensis to 87% in L. multiflorum. Of 40 amplified markers, fourteen (35%) produced species-difference alleles in the relation to cultivars used in the present study. Thirty-five LPSSR locus-derived alleles were found to be specific to Lolium species, 4 to F. pratensis and 6 to F. arundinacea. For LMSSR alleles, 8 were specific to Lolium species and 5 were only associated with L. multiflorum, and null alleles were detected for F. pratensis in all instances. These species-difference markers could clearly identify different accessions of Festulolium. Cluster analysis separated the individual taxa and showed grouping of intergeneric hybrids based on genomic composition. The data distinguished between the species and reflected the known pedigree of the cultivars and the differences between the species. The dendrogram also distinguished between the Festulolium accessions and clearly demonstrated the relations between Festulolium hybrids and their parent species.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/j.1439-0523.2004.00962.x