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Hokkaido University Collection of Scholarly and Academic Papers >
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Development of a new device for artificial insemination in cynomolgus macaques
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| Title: | Development of a new device for artificial insemination in cynomolgus macaques |
| Authors: | KOYAMA, Shuzo Browse this author | | FUKUDA, Koji Browse this author | | WATANABE, Sho Browse this author | | KOHARA, Sakae Browse this author | | TSUCHIYA, Hideaki Browse this author →KAKEN DB | | FUKUZAKI, Koichiro Browse this author | | NAGANO, Masashi Browse this author →KAKEN DB | | UNO, Yasuhiro Browse this author | | HOSOI, Yoshihiko Browse this author →KAKEN DB |
| Keywords: | Artificial insemination | | Cynomolgus monkey | | Device | | Semen |
| Issue Date: | Oct-2016 |
| Publisher: | 日本繁殖生物学会 |
| Journal Title: | Journal of Reproduction and Development |
| Volume: | 62 |
| Issue: | 5 |
| Start Page: | 527 |
| End Page: | 529 |
| Publisher DOI: | 10.1262/jrd.2016-045 |
| Abstract: | In cynomolgus macaques, an important animal species for biomedical research, efficient reproduction has been hampered partly due to the difficulties of artificial insemination (AI) using straw tubes developed for humans or farm animals, because cynomolgus macaques have a complex cervical canal structure. In this study, taking into consideration the unique structure of the macaque cervical canal, we developed a novel device for AI, comprised of a syringe and an outer cylinder. At 24 and 48 h after using this device to inject semen into one female, viable sperm were observed in the oviduct where the sperm meets the oocytes. We then attempted AI using this new device on 10 females that were at pre-ovulation, and pregnancy was successful in three animals (30% pregnancy rate). These results show that the newly developed device can be used for AI in cynomolgus macaques. |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63196 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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