An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)

Sato, Kousuke; Kawamoto, Kyoji; Shimamura, Shintaro; Ichikawa, Satoshi; Matsuda, Akira

doi:10.1016/j.bmcl.2016.10.040


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An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)

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Title:	An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)
Authors:	Sato, Kousuke Browse this author →KAKEN DB
	Kawamoto, Kyoji Browse this author
	Shimamura, Shintaro Browse this author
	Ichikawa, Satoshi Browse this author →KAKEN DB
	Matsuda, Akira Browse this author →KAKEN DB
Keywords:	Epigenetic
	Nucleic acids
	5-Formyl-2 '-deoxycytidine
	Covalent bond
	DNA methyltransferase
Issue Date:	15-Nov-2016
Publisher:	Elsevier
Journal Title:	Bioorganic & Medicinal Chemistry Letters
Volume:	26
Issue:	22
Start Page:	5395
End Page:	5398
Publisher DOI:	10.1016/j.bmcl.2016.10.040
PMID:	27780634
Abstract:	5-Methylcytosine (mC) is known to induce epigenetic changes. Ten-eleven translocation (TET) enzymes produce the further oxidized 5-substituted cytosine derivatives, 5-formylcytosine (fC) and 5-carboxylcytosine (caC). However, their roles are unclear thus far. Here, we synthesized oligodeoxyribonucleotides (ODNs) containing 5-formyl-2'-deoxycytidine and examined their interactions with DNA cytosine-5 methyltransferase (DNMT). We found that the ODN sequence containing fCpG formed a covalent complex with both bacterial and mouse recombinant DNMTs in the absence of any cofactors. The covalent bonding with DNMT suggests that the fCpG sequence in DNA may play a role in epigenetic regulation. (C) 2016 Elsevier Ltd. All rights reserved.
Rights:	© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Rights:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Type:	article (author version)
URI:	http://hdl.handle.net/2115/71972
Appears in Collections:	薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤浩輔

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