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Severe Skin Permeability Barrier Dysfunction in Knockout Mice Deficient in a Fatty Acid omega-Hydroxylase Crucial to Acylceramide Production
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| Title: | Severe Skin Permeability Barrier Dysfunction in Knockout Mice Deficient in a Fatty Acid omega-Hydroxylase Crucial to Acylceramide Production |
| Authors: | Miyamoto, Masatoshi Browse this author | | Itoh, Narumi Browse this author | | Sawai, Megumi Browse this author | | Sassa, Takayuki Browse this author →KAKEN DB | | Kihara, Akio Browse this author →KAKEN DB |
| Issue Date: | Feb-2020 |
| Publisher: | Elsevier |
| Journal Title: | Journal of investigative dermatology |
| Volume: | 140 |
| Issue: | 2 |
| Start Page: | 319 |
| End Page: | 326 |
| Publisher DOI: | 10.1016/j.jid.2019.07.689 |
| Abstract: | The skin permeability barrier is indispensable for maintaining water inside the body and preventing the invasion of pathogens and allergens; abnormalities lead to skin disorders such as atopic dermatitis and ichthyosis. Acylceramide is an essential lipid for skin barrier formation, and CYP4F22 is a fatty acid omega-hydroxylase involved in its synthesis. Mutations in CYP4F22 cause autosomal recessive congenital ichthyosis, although the symptoms vary among mutation sites and types. Here, we generated knockout mice deficient in Cyp4f39, the mouse ortholog of human CYP4F22, to investigate the effects of completely abrogating the function of the fatty acid omega-hydroxylase involved in acylceramide production on skin barrier formation. Cyp4f39 knockout mice died within 8 hours of birth. Large increases in transepidermal water loss and penetration of a dye from outside the body were observed, indicating severe skin barrier dysfunction. Histologic analyses of the epidermis revealed impairment of lipid lamella formation, accumulation of corneodesmosomes in the stratum corneum, and persistence of periderm. In addition, lipid analyses by mass spectrometry showed almost complete loss of acylceramide and its precursor omega-hydroxy ceramide. In conclusion, our findings provide clues to the molecular mechanisms of skin barrier abnormalities and the pathogenesis of ichthyosis caused by Cyp4f39 and CYP4F22 by association. |
| Rights: | ©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Publisher URI: | https://www.jidonline.org/article/S0022-202X(19)32557-6/fulltext |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/80502 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木原 章雄
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