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マルチオミクス解析による好熱性水素酸化細菌からの可逆的TCA 回路の発見
布浦, 拓郎;力石, 嘉人;跡見, 晴幸
Permalink : http://hdl.handle.net/2115/81349
JaLCDOI : 10.14943/lowtemsci.79.31
KEYWORDS : 好熱菌;TCA 回路;代謝解析;安定同位体;Thermophile;TCA cycle;Metabolomics;Stable isotope
Abstract
生物による炭素固定は地球上の生命そして生態系の維持において必須の機能であり，還元的TCA回路（reductive tricarboxylic acid cycle）は，ウッドリュンガル（Wood-Ljungdahl）経路（アセチルCoA 経路）と並び，最も始原的な炭酸固定経路ではないかと考えられてきた．我々は始原的系統群に属す好熱性水素酸化細菌Thermosulfidibacter takaii に対するマルチオミクス解析を実施し，二酸化炭素や有機酸等，入手可能な炭素源により機能方向が変化する可逆的TCA 回路を発見した．更に，このTCA 回路の性質を基に，初期生命が還元的TCA 回路でなく，可逆的TCA 回路を保持した可能性を提唱した．
Inorganic carbon fixation is essential to sustain life and ecosystem on Earth. The reductive tricarboxylic acid (rTCA) cycle, also known as the citric acid cycle, and the Wood-Ljungdahl pathway, also known as the acetyl-CoA pathway, are the most ancient carbon fixation metabolisms. A combination of multi-omics analyses revealed a previously unknown reversible TCA cycle whose direction was regulated by available carbon sources such as inorganic carbon and organic acids. Moreover, we hypothesized that primordial life harbored the reversible TCA cycle but not rTCA cycle.
Inorganic carbon fixation is essential to sustain life and ecosystem on Earth. The reductive tricarboxylic acid (rTCA) cycle, also known as the citric acid cycle, and the Wood-Ljungdahl pathway, also known as the acetyl-CoA pathway, are the most ancient carbon fixation metabolisms. A combination of multi-omics analyses revealed a previously unknown reversible TCA cycle whose direction was regulated by available carbon sources such as inorganic carbon and organic acids. Moreover, we hypothesized that primordial life harbored the reversible TCA cycle but not rTCA cycle.
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