Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2

Nagai, Kazuhiko; Takikawa, Osamu; Kawakami, Naoko; Fukao, Mitsuhiro; Soma, Takamitsu; Oda, Atsushi; Nishiya, Tadashi; Hayashi, Misato; Lu, Lingyun; Nakano, Manabu; Kajita, Emi; Fujita, Hiroyoshi; Miwa, Soichi

Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2

Nagai, Kazuhiko; Takikawa, Osamu; Kawakami, Naoko et al.

Archives of Biochemistry and Biophysics, 2006, 452(1), 29-37

Permalink : https://hdl.handle.net/2115/14620

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アクセス権	open access
URI	https://hdl.handle.net/2115/14620
タイトル	Cloning and functional characterization of a novel up-regulator, cartregulin, of carnitine transporter, OCTN2
著者	Nagai, Kazuhiko ; Takikawa, Osamu ; Kawakami, Naoko ; Fukao, Mitsuhiro ; Soma, Takamitsu ; Oda, Atsushi ; Nishiya, Tadashi ; Hayashi, Misato ; Lu, Lingyun ; Nakano, Manabu ; Kajita, Emi ; Fujita, Hiroyoshi ; Miwa, Soichi
著者名 Nagai, Kazuhiko
著者名 Takikawa, Osamu
著者名 Kawakami, Naoko
著者名 Fukao, Mitsuhiro
著者名 Soma, Takamitsu
著者名 Oda, Atsushi
著者名 Nishiya, Tadashi
著者名 Hayashi, Misato
著者名 Lu, Lingyun
著者名 Nakano, Manabu
著者名 Kajita, Emi
著者名 Fujita, Hiroyoshi
e-Rad_Researcher 40157706 著者名 Miwa, Soichi
言語	英語
キーワード	cDNA cloning
	Acetylcarnitine
	Carnitine
	OCTN2
	mRNA stabilization
	Transporter
	Up-regulation
	Alzheimer’s disease
発行日	2006-08-01
出版者	Elsevier Inc.
収録物名	Archives of Biochemistry and Biophysics
巻(号)	452(1)
ページ	29-37
抄録	Acetylcarnitine exerts therapeutic effects on some neurological disorders including Alzheimer’s disease. OCTN2 is known as a transporter for acetylcarnitine, but its expression in the brain is very low. To examine a brain-specific transporter for acetylcarnitine, we screened a rat brain cDNA library by hybridization using a DNA probe conserved among an OCTN family. A cDNA homologous to OCTN2 cDNA was isolated. The cDNA encoded a novel 146-amino acid protein with one putative transmembrane domain. The mRNA was expressed not only in rat brain but also in some other tissues. The novel protein was localized in endoplasmic reticulum when expressed in COS-7 cells but exhibited no transport activity for acetylcarnitine. However, when co-expressed with OCTN2, it enhanced the OCTN2-mediated transport by about twofold. The enhancement was accompanied by an increase in the levels of mRNA and protein. When OCTN2 was expressed in Xenopus oocytes by injection of its cRNA, its transport activity was enhanced by co-expression of the novel protein. These data suggest that the novel protein increases OCTN2 by stabilizing the mRNA in endoplasmic reticulum. The protein may be an up-regulator of OCTN2 and is tentatively designated cartregulin.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0003-9861
EISSN	1096-0384
関連情報	PMID 16839516
関連情報	URI http://www.sciencedirect.com/science/journal/00039861
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.abb.2006.06.006