Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.

Yokouchi, Hiroshi; Yamazaki, Koichi; Asahina, Hajime; Shigemura, Masahiko; Moriyama, Takanori; Takaoka, Kazuo; Moriya, Jun; Itoh, Tomoo; Kinoshita, Ichiro; Dosaka-Akita, Hirotoshi; Tsutsumi, Yutaka; Nishimura, Masaharu

Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.

Yokouchi, Hiroshi; Yamazaki, Koichi; Asahina, Hajime et al.

2006, 26(4B), 2821-2827

Permalink : https://hdl.handle.net/2115/15843

このアイテムのアクセス数:1,566件（2026-05-18 02:47 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
AR26-4B	pdf	615 KB	1,522

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/15843
タイトル	Establishment and characterization of amylase-producing lung adenocarcinoma cell line, IMEC-2.
著者	Yokouchi, Hiroshi ; Yamazaki, Koichi ; Asahina, Hajime ; Shigemura, Masahiko ; Moriyama, Takanori ; Takaoka, Kazuo ; Moriya, Jun ; Itoh, Tomoo ; Kinoshita, Ichiro ; Dosaka-Akita, Hirotoshi ; Tsutsumi, Yutaka ; Nishimura, Masaharu
著者名 Yokouchi, Hiroshi
著者名 Yamazaki, Koichi
著者名 Asahina, Hajime
著者名 Shigemura, Masahiko
著者名 Moriyama, Takanori
著者名 Takaoka, Kazuo
著者名 Moriya, Jun
著者名 Itoh, Tomoo
著者名 Kinoshita, Ichiro
著者名 Dosaka-Akita, Hirotoshi
著者名 Tsutsumi, Yutaka
著者名 Nishimura, Masaharu
言語	英語
キーワード	amylase
	lung cancer
	cell line
	sialylation
	IMEC-2
発行日	2006-07
巻(号)	26(4B)
ページ	2821-2827
抄録	Background: Few studies have successfully established an amylase-producing lung cancer cell line or have examined its cytological, biochemical and biological features. Materials and Methods: Cancer cells isolated from pleural effusion using a gradient method were cultivated. Results: Amylase production from the newly established cell line was confirmed by positive staining for α-amylase and increased amylase levels in culture supernatant. Electron microscopy revealed zymogen granule-like structures. Sialylation of salivary-type amylase was confirmed directly from the cell line by examining neuraminidase sensitivity and amylase elution profile under high-performance liquid chromatography. EGFR or KRAS mutation was not found. Conclusions: This cell line offers a useful tool for analyzing the pathogenesis and pathophysiology of amylase-producing lung cancers. Moreover, it might be useful for probing metastasis and invasiveness of lung cancer cells and for developing an early diagnostic method based on sialylated salivary-amylase production.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	0250-7005
関連情報	PMID 16886600
関連情報	URI http://www.amedeo.com/medicine/ln/ln4.htm