Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography

Ando, Yasuhiro; Haba, Yusuke; Takase, Kiwamu; Sakai, Shinya

Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography

Ando, Yasuhiro; Haba, Yusuke; Takase, Kiwamu et al.

北海道大学水産科学研究彙報, 2007, 57(3), 37-41

Permalink : https://hdl.handle.net/2115/32486

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アクセス権	open access
URI	https://hdl.handle.net/2115/32486
タイトル	Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography
著者	Ando, Yasuhiro ; Haba, Yusuke ; Takase, Kiwamu ; Sakai, Shinya
e-Rad_Researcher 30261340 著者名 Ando, Yasuhiro
著者名 Haba, Yusuke
著者名 Takase, Kiwamu
著者名 Sakai, Shinya
言語	英語
キーワード	Regiospecific analysis
	High-performance thin-layer chromatography
	Triacylglycerol
	Fatty acid
	Fish oil
	Seal oil
発行日	2007-12
出版者	北海道大学大学院水産科学研究院
出版者	RESEARCH FACULTY OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
収録物名	北海道大学水産科学研究彙報
収録物名	BULLETIN OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
巻(号)	57(3)
ページ	37-41
抄録	This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG.
資源タイプ	紀要論文
出版タイプ	Version of Record
PISSN	1346-1842