Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.

Yasui, George Shigueki; Arias-Rodriguez, Lenin; Fujimoto, Takafumi et al.

CryoLetters, 2008, 29(5), 383-390

Permalink : https://hdl.handle.net/2115/39128

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アクセス権
open access
URI
https://hdl.handle.net/2115/39128
タイトル
Simple and inexpensive method for cryopreservation of fish sperm combining straw and powdered dry ice.
著者
Yasui, George Shigueki ; Arias-Rodriguez, Lenin ; Fujimoto, Takafumi ; Arai, Katsutoshi
著者名
Yasui, George Shigueki
著者名
Arias-Rodriguez, Lenin
著者名
Fujimoto, Takafumi
e-Rad_Researcher
00137902
著者名
Arai, Katsutoshi
言語
英語
キーワード
cryopreservation
dry ice
fish
cooling rate
Misgurnus
sperm
straw
発行日
2008-09-01
出版者
CryoLetters
収録物名
CryoLetters
巻(号)
29(5)
ページ
383-390
抄録
Here, we propose a simple and inexpensive method for fish sperm cryopreservation. Sperm samples of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) were diluted 7-fold by an extender containing 63.5mM NaCl, 114mM KCl, 20mM Tris and 10% methanol. The cryogenic straws were placed in three kinds of self-made tubes which diameter was changed by commercially available materials and then immersed into powdered dry ice for 2 min and plunged into liquid nitrogen. This procedure resulted in a cooling rate at -421.4±119.84 (control), -55.8 ± 4.32 (tube 1), -40.2 ± 3.43 (tube 2) and -33.3 ± 2.09ºC/min (tube 3). In the slowest cooling rate by the tube 3, total motility (72 ± 3%), duration (146 ± 12s) and hatching rates (29 ± 04%) were higher than those by other rates. Progressive motility (83±5%) did not differ significantly from fresh samples.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0143-2044
EISSN
1742-0644
NCID
AA00145687
関連情報
PMID
18946552