Targeted sequence alteration of a chromosomal locus in mouse liver

Kamiya, Hiroyuki; Uchiyama, Masayuki; Piao, Jingshu et al.

International Journal of Pharmaceutics, 2010, 387(1-2), 180-183

Permalink : https://hdl.handle.net/2115/42890

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アクセス権
open access
URI
https://hdl.handle.net/2115/42890
タイトル
Targeted sequence alteration of a chromosomal locus in mouse liver
著者
Kamiya, Hiroyuki ; Uchiyama, Masayuki ; Piao, Jingshu ; Nakatsu, Yoshimichi ; Tsuzuki, Teruhisa ; Harashima, Hideyoshi
著者名
Kamiya, Hiroyuki
著者名
Uchiyama, Masayuki
著者名
Piao, Jingshu
著者名
Nakatsu, Yoshimichi
著者名
Tsuzuki, Teruhisa
e-Rad_Researcher
00183567
著者名
Harashima, Hideyoshi
言語
英語
キーワード
Targeted sequence alteration
Gene correction
Tailed duplex DNA fragment
rpsL transgenic mouse
Hydrodynamic tail vein injection
発行日
2010-03-15
出版者
Elsevier B.V.
収録物名
International Journal of Pharmaceutics
巻(号)
387(1-2)
ページ
180-183
抄録
Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means of hydrodynamic tail vein injection into liver of transgenic mouse harboring a reporter gene (the rpsL gene) in its genome. The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene. The anticipated T → G sequence alteration was induced in the transgene in the liver with an efficiency of ∼0.1%. These results demonstrate the significant potential of this method for applications in gene therapy and biotechnology.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
PISSN
0378-5173
関連情報
PMID
20025952
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.ijpharm.2009.12.020