Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis

Yamada, Shuhei; Onishi, Masako; Fujinawa, Reiko; Tadokoro, Yuko; Okabayashi, Koji; Asashima, Makoto; Sugahara, Kazuyuki

Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis

Yamada, Shuhei; Onishi, Masako; Fujinawa, Reiko et al.

Glycobiology, 2009, 19(5), 488-498

Permalink : https://hdl.handle.net/2115/43036

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/43036
タイトル	Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis
著者	Yamada, Shuhei ; Onishi, Masako ; Fujinawa, Reiko ; Tadokoro, Yuko ; Okabayashi, Koji ; Asashima, Makoto ; Sugahara, Kazuyuki
e-Rad_Researcher 70240017 著者名 Yamada, Shuhei
著者名 Onishi, Masako
著者名 Fujinawa, Reiko
著者名 Tadokoro, Yuko
著者名 Okabayashi, Koji
著者名 Asashima, Makoto
e-Rad_Researcher 60154449 著者名 Sugahara, Kazuyuki
言語	英語
キーワード	Xenopus laevis
	chondroitin sulfate
	heparan sulfate
	glycosaminoglycan
	embryogenesis
発行日	2009-05
出版者	Oxford University Press
収録物名	Glycobiology
巻(号)	19(5)
ページ	488-498
抄録	Xenopus laevis is an excellent animal for analyzing early vertebrate development. Various effects of glycosaminoglycans (GAGs) on growth factor-related cellular events during embryogenesis have been demonstrated in Xenopus. To elucidate the relationship between alterations in fine structure and changes in the specificity of growth factor-binding during Xenopus development, heparan sulfate (HS) and chondroitin/dermatan sulfate (CS/DS) chains were isolated at four different embryonic stages and their structure and growth factor-binding capacities were compared. The total amounts of both HS and CS/DS chains decreased from the pre-midblastula transition to the gastrula stage, but increased exponentially during the following developmental stages. The length of HS chains was not significantly affected by development, whereas that of CS/DS chains increased with development. The disaccharide composition of GAGs in embryos also changed during development. The degree of sulfation of the HS chains gradually decreased with development. The predominant sulfation position in the CS/DS chains shifted from C4 to C6 of GalNAc during embryogenesis. Growth factor-binding experiments using a BIAcore system demonstrated that GAGs bound growth factors including fibroblast growth factors-1, and -2, midkine, and pleiotrophin, with comparable affinities. These affinities significantly varied during development, although the correlation between the structural alterations of GAGs and the change in the ability to bind growth factors remains to be clarified. Expression of saccharide sequences, which specifically interact with a growth factor, might be regulated during development.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Glycobiology following peer review. The definitive publisher-authenticated version 19(5):488-498, May 2009 is available online at: http://dx.doi.org/10.1093/glycob/cwp005
PISSN	0959-6658
EISSN	1460-2423
関連情報	PMID 19190026
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/glycob/cwp005