Rapid Preparation of Fluorescent 9-Anthrylmethyl Esters for Fatty Acid Analysis of Small Amount of Triacylglycerols

Ando, Yasuhiro; Haba, Yusuke; Soma, Kazutaka; Hiraoka, Yuko; Takatsu, Tetsuya

Rapid Preparation of Fluorescent 9-Anthrylmethyl Esters for Fatty Acid Analysis of Small Amount of Triacylglycerols

Ando, Yasuhiro; Haba, Yusuke; Soma, Kazutaka et al.

Lipids, 2007, 42(10), 955-960

Permalink : https://hdl.handle.net/2115/44613

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/44613
タイトル	Rapid Preparation of Fluorescent 9-Anthrylmethyl Esters for Fatty Acid Analysis of Small Amount of Triacylglycerols
著者	Ando, Yasuhiro ; Haba, Yusuke ; Soma, Kazutaka ; Hiraoka, Yuko ; Takatsu, Tetsuya
e-Rad_Researcher 30261340 著者名 Ando, Yasuhiro
著者名 Haba, Yusuke
著者名 Soma, Kazutaka
著者名 Hiraoka, Yuko
e-Rad_Researcher 50241378 著者名 Takatsu, Tetsuya
言語	英語
キーワード	9-Anthrylmethyl ester
	Fatty acid
	Fish oil
	HPLC
	Transesterification
	Triacylglycerol
発行日	2007-10
収録物名	Lipids
巻(号)	42(10)
ページ	955-960
抄録	This paper proposes a one-step method for preparation of fluorescent 9-anthrylmethyl esters from triacylglycerols (TAG) ranging in amount from 0.1 to 5 μg. It involves base-catalyzed transesterification using potassium 9-anthracenemethoxide, prepared by proton exchange between 9-anthracenemethanol and potassium tert-butoxide. Transesterification for 10 min at room temperature gave the fatty acid 9-anthrylmethyl esters in nearly maximal yields (82–85%). The products could be analyzed by reversed-phase HPLC without purification. Excellent linear relationships were observed for standard curves of 10–250 pmol of TAG standards (16:0, 19:0, 18:2 and 22:6), and differences in the slopes were less than 5% among the standards. Almost consistent compositions of the esters were observed for the products formed from 0.5 to 5 μg or less of fish oils TAG, and they were similar to those obtained by HPLC of ordinary multi-step synthesis products and by GLC of methyl esters. The present method is a great improvement of derivatization time, and is powerful for fatty acid analysis of small amounts of natural TAG.
資源タイプ	学術雑誌論文
出版タイプ	Submitted Manuscript Under Review
権利情報	The original publication is available at www.springerlink.com
PISSN	0024-4201
EISSN	1558-9307
関連情報	PMID 17694342
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s11745-007-3100-2