The Oligomeric States of the Photosystems and the Light-Harvesting Complexes in the Chl b-Less Mutant

Takabayashi, Atsushi; Kurihara, Katsunori; Kuwano, Masayoshi; Kasahara, Yasuhiro; Tanaka, Ryouichi; Tanaka, Ayumi

The Oligomeric States of the Photosystems and the Light-Harvesting Complexes in the Chl b-Less Mutant

Takabayashi, Atsushi; Kurihara, Katsunori; Kuwano, Masayoshi et al.

Plant and Cell Physiology, 2011, 52(12), 2103-2114

Permalink : https://hdl.handle.net/2115/50292

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アクセス権	open access
URI	https://hdl.handle.net/2115/50292
タイトル	The Oligomeric States of the Photosystems and the Light-Harvesting Complexes in the Chl b-Less Mutant
著者	Takabayashi, Atsushi ; Kurihara, Katsunori ; Kuwano, Masayoshi ; Kasahara, Yasuhiro ; Tanaka, Ryouichi ; Tanaka, Ayumi
e-Rad_Researcher 90546417 著者名 Takabayashi, Atsushi
著者名 Kurihara, Katsunori
著者名 Kuwano, Masayoshi
e-Rad_Researcher 20273849 著者名 Kasahara, Yasuhiro
e-Rad_Researcher 20311516 著者名 Tanaka, Ryouichi
e-Rad_Researcher 10197402 著者名 Tanaka, Ayumi
言語	英語
キーワード	Arabidopsis thaliana
	Blue-native-PAGE
	Chl b-less plant
	Photosystem
発行日	2011-12
出版者	Oxford University Press
収録物名	Plant and Cell Physiology
巻(号)	52(12)
ページ	2103-2114
抄録	The reversible associations between the light-harvesting complexes (LHCs) and the core complexes of PSI and PSII are essential for the photoacclimation mechanisms in higher plants. Two types of chlorophylls, chlorophyll a and chlorophyll b, both function in light harvesting and are required for the biogenesis of the photosystems. Chlorophyll b-less plants have been studied to determine the function of the LHCs because the chlorophyll b deficiency has severe effects specific to the LHCs. Previous studies have shown that the amounts of the LHCs, especially the LHCII trimer, were decreased in the mutants; however, it is still unclear whether chlorophyll b is required for the assembly of the LHCs and for the association of the LHCs with PSI and PSII. Here, to reveal the function of chlorophyll b in the LHCs, we investigated the oligomeric states of the LHCs, PSI and PSII in the Arabidopsis chlorophyll b-less mutant. A two-dimensional blue native-PAGE/SDS-PAGE demonstrated that the PSI-LHCI supercomplex was fully assembled in the absence of chlorophyll b, whereas the trimeric LHCII and PSII-LHCII supercomplexes were not detected. The PSI-NAD(P)H dehydrogenase (NDH) supercomplexes were also assembled in the mutant. Furthermore, we detected two forms of monomeric LHC proteins. The faster migrating forms, which were detected primarily in the mutant, were likely apo-LHC proteins, whereas the slower migrating forms were likely the LHC proteins that contained chlorophyll a. These findings increase our understanding of the chlorophyll b function in the assembly of LHCs and the association of the LHCs with PSI, PSII and NDH.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The definitive publisher-authenticated version Plant Cell Physiol (2011) 52 (12): 2103-2114 is available online at: http://pcp.oxfordjournals.org/content/52/12/2103
PISSN	0032-0781
関連情報	PMID 22006940
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/pcp/pcr138