Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus

Takano, Ayako; Yoshii, Kentaro; Omori-Urabe, Yuki; Yokozawa, Kana; Kariwa, Hiroaki; Takashima, Ikuo

Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus

Takano, Ayako; Yoshii, Kentaro; Omori-Urabe, Yuki et al.

Archives of Virology, 2011, 156(11), 1931-1941

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/50373
タイトル	Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus
著者	Takano, Ayako ; Yoshii, Kentaro ; Omori-Urabe, Yuki ; Yokozawa, Kana ; Kariwa, Hiroaki ; Takashima, Ikuo
著者名 Takano, Ayako
e-Rad_Researcher 50421988 著者名 Yoshii, Kentaro
著者名 Omori-Urabe, Yuki
著者名 Yokozawa, Kana
e-Rad_Researcher 70224714 著者名 Kariwa, Hiroaki
e-Rad_Researcher 30002083 著者名 Takashima, Ikuo
言語	英語
発行日	2011-11
出版者	Springer Vienna
収録物名	Archives of Virology
巻(号)	156(11)
ページ	1931-1941
抄録	Tick-borne encephalitis virus (TBEV) causes severe encephalitis in humans. The Sofjin-HO strain is the prototype strain of the TBEV Far-Eastern subtype and is highly pathogenic in a mouse model. In this study, we constructed replicons and infectious cDNA clones of the Sofjin-HO strain. The replication of the replicon RNA was confirmed, and infectious viruses were recovered from the infectious cDNA clone. The recombinant viruses showed similar virulence characteristics to those of the parental virus. While characterizing the replicon and infectious cDNA, several amino acid differences derived from cell culture adaptations were analysed. The amino acids differences at E position 496 and NS4A position 58 were found to affect viral replication. The Gly- or Ala-to-Glu substitution at E position 122 was shown to increase neuroinvasiveness in mice. These replicons and infectious cDNA clones are useful in revealing the viral molecular determinant involved in the replication and pathogenicity of TBEV.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The original publication is available at www.springerlink.com
PISSN	0304-8608
関連情報	PMID 21785855
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s00705-011-1066-0