Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 cells

Sun, Yongkun; Guo, Zhikun; Iku, Shouhei; Saito, Takeshi; Kurasaki, Masaaki

Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 cells

Sun, Yongkun; Guo, Zhikun; Iku, Shouhei et al.

Journal of Applied Toxicology, 2013, 33(12), 1484-1492

Permalink : https://hdl.handle.net/2115/53421

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/53421
タイトル	Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 cells
著者	Sun, Yongkun ; Guo, Zhikun ; Iku, Shouhei ; Saito, Takeshi ; Kurasaki, Masaaki
著者名 Sun, Yongkun
著者名 Guo, Zhikun
著者名 Iku, Shouhei
e-Rad_Researcher 40153811 著者名 Saito, Takeshi
e-Rad_Researcher 80161727 著者名 Kurasaki, Masaaki
言語	英語
キーワード	apoptosis
	DNA methylation
	endocrine disrupter
	epigenetic
	Sir2
発行日	2013-12
出版者	John Wiley & Sons
収録物名	Journal of Applied Toxicology
巻(号)	33(12)
ページ	1484-1492
抄録	Diethyl phthalate (DEP) works as a phthalate plasticizer and is ubiquitously used in personal care products, cosmetics, medical equipment and pharmaceutical coating. DEP is considered a potential endocrine disruptor. Previously we found DEP-enhanced apoptosis induced by serum deprivation in PC12 cells. However, the relationship between DEP and longevity-related factors, sirtuins and epigenetic factors (e.g. DNA methyltransferases) remains unclear, because genome modification caused by chemical toxicity, sirtuins and epigenetic factors have played key roles in abnormal metabolism and development. Here, we investigate whether DEP affects sirtuins (SIRT1 and SIRT2) and methyltranferases (DNMT1 and DNMT3a) on the apoptosis of PC12 cells. We found that DNMT3a was significantly decreased by serum deprivation. However, DNMT3a, DNMT3b and SIRT1 were significantly increased under the enhancement of apoptosis induced by serum deprivation. These results suggest that SIRT1, DNMT3a and DNMT3b play multiple and complex roles in different apoptotic stages. Our results showed DEP triggered epigenetic factors on PC12 cells apoptosis under nutrition stress. Finally, our results suggest that monitoring epigenetic factors such as DNMT3a, DNMT3b and SIRT1 could be a useful tool for chemical toxicity risk assessment.
資源タイプ	学術雑誌論文
出版タイプ	Submitted Manuscript Under Review
権利情報	Copyright © 2012 John Wiley & Sons, Ltd. Published online in Wiley Online Library: 11 September 2012
PISSN	0260-437X
NCID	AA10635955
関連情報	PMID 22968747
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/jat.2816