Determination of the functional domain of a mouse autonomous replicating sequence.

Hayashi, Chiharu; Fujino, Hiromichi; Ogata, Masanori et al.

Biological & pharmaceutical bulletin, 1997, 20(6), 690-693

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アクセス権
open access
URI
https://hdl.handle.net/2115/53973
タイトル
Determination of the functional domain of a mouse autonomous replicating sequence.
著者
Hayashi, Chiharu ; Fujino, Hiromichi ; Ogata, Masanori ; Sato, Yoshinori ; Iguchi-Ariga, Sanae M. M. ; Ariga, Hiroyoshi
著者名
Hayashi, Chiharu
著者名
Fujino, Hiromichi
著者名
Ogata, Masanori
著者名
Sato, Yoshinori
e-Rad_Researcher
90184283
著者名
Iguchi-Ariga, Sanae M. M.
e-Rad_Researcher
20143505
著者名
Ariga, Hiroyoshi
言語
英語
キーワード
ARS
c-myc
replication
発行日
1997-06
出版者
The Pharmaceutical Society of Japan
収録物名
Biological & pharmaceutical bulletin
巻(号)
20(6)
ページ
690-693
抄録
We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BgIII to EcoRI sites cloned as ARS was 2658 bp long. There exist three interesting domains: a TA repeat, a myc like box (essential sequence for c-myc ARS), and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hygromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0918-6158
関連情報
PMID
9212992
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1248/bpb.20.690