A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis

Uji, Toshiki; Hirata, Ryo; Fukuda, Satoru; Mizuta, Hiroyuki; Saga, Naotsune

A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis

Uji, Toshiki; Hirata, Ryo; Fukuda, Satoru et al.

Marine Biotechnology, 2014, 16(3), 251-255

Permalink : https://hdl.handle.net/2115/57285

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/57285
タイトル	A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis
著者	Uji, Toshiki ; Hirata, Ryo ; Fukuda, Satoru ; Mizuta, Hiroyuki ; Saga, Naotsune
e-Rad_Researcher 00760597 著者名 Uji, Toshiki
著者名 Hirata, Ryo
著者名 Fukuda, Satoru
e-Rad_Researcher 00250499 著者名 Mizuta, Hiroyuki
e-Rad_Researcher 10231333 著者名 Saga, Naotsune
言語	英語
キーワード	Pyropia yezoensis
	Red alga
	Selection marker
	Stable transformation
発行日	2014-06
出版者	Springer
収録物名	Marine Biotechnology
巻(号)	16(3)
ページ	251-255
抄録	Marine macroalgae play an important role in marine coastal ecosystems and are widely used as sea vegetation foodstuffs and for industrial purposes. Therefore, there have been increased demands for useful species and varieties of these macroalgae. However, genetic transformation in macroalgae has not yet been established. We have developed a dominant selection marker for stable nuclear transformation in the red macroalga Pyropia yezoensis. We engineered the coding region of the aminoglycoside phosphotransferase gene aph7aEuro(3) from Streptomyces hygroscopicus to adapt codon usage of the nuclear genes of P. yezoensis. We designated this codon-optimized aph7aEuro(3) gene as PyAph7. After bombarding P. yezoensis cells with plasmids containing PyAph7 under the control of their endogenous promoter, 1.9 thalli (or individuals) of hygromycin-resistant strains were isolated from a 10-mm square piece of the bombarded thallus. These transformants were stably maintained throughout the asexual life cycle. Stable expression of PyAph7was verified using Southern blot analysis and genomic PCR and RT-PCR analyses. PyAph7 proved to be a new versatile tool for stable nuclear transformation in P. yezoensis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	The final publication is available at link.springer.com
PISSN	1436-2228
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1007/s10126-013-9549-5