open access

https://hdl.handle.net/2115/58046

Polyphosphate accumulation is driven by transcriptome alterations that lead to near-synchronous and near-equivalent uptake of inorganic cations in an arbuscular mycorrhizal fungus

英語

transcriptome

2014-11

Blackwell Publishing

New Phytologist

204(3)

638-649

・Arbuscular mycorrhizal (AM) fungi accumulate a massive amount of phosphate as polyphosphate to deliver to the host, but the physiological and molecular mechanisms underlying have yet to be elucidated. In the present study, the dynamics of cationic components during polyphosphate accumulation were investigated in conjunction with transcriptome analysis. ・Rhizophagus sp. HR1 was grown with Lotus japonicus under phosphorus-deficient conditions, and extraradical mycelia were harvested after phosphate application at prescribed intervals. Levels of polyphosphate, inorganic cations, and amino acids were measured, and RNA-Seq was performed on the Illumina platform. ・Phosphate application triggered not only polyphosphate accumulation but also near-synchronous and -equivalent uptake of Na+, K+, Ca2+, and Mg2+, whereas no distinct changes in the levels of amino acids were observed. During polyphosphate accumulation, genes responsible for mineral uptake, phosphate and nitrogen metabolisms, and maintenance of cellular homeostasis were up-regulated. ・The results suggest that the inorganic cations play a major role in neutralizing negative charge of polyphosphate, and these processes are achieved by the orchestrated regulation of gene expression. Our findings for the first time provide a global picture of the cellular responses to increased phosphate availability, which is the initial process of nutrient delivery in the associations.

学術雑誌論文

Accepted Manuscript

The definitive version is available at www.blackwellsynergy.com

0028-646X