open access

https://hdl.handle.net/2115/62999

Fluorescence lifetime measurements in heterogeneous scattering medium

英語

time-resolved method

2016-07

Society of Photo-Optical Instrumentation Engineers (SPIE)

Journal of biomedical optics

21(7)

075013

Fluorescence lifetime in heterogeneous multiple light scattering systems is analyzed by an algorithm without solving the diffusion or radiative transfer equations. The algorithm assumes that the optical properties of medium are constant in the excitation and emission wavelength regions. If the assumption is correct and the fluorophore is a single species, the fluorescence lifetime can be determined by a set of measurements of temporal point-spread function of the excitation light and fluorescence at two different concentrations of the fluorophore. This method is not dependent on the heterogeneity of the optical properties of the medium as well as the geometry of the excitation-detection on an arbitrary shape of the sample. The algorithm was validated by an indocyanine green fluorescence in phantom measurements and demonstrated by an in vivo measurement.

学術雑誌論文

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Creative Commons Attribution 3.0 Unported

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.　”Fluorescence lifetime measurements inheterogeneous scattering medium,” J. Biomed. Opt. 21(7), 075013 (2016)

1083-3668