Differential expression of endothelial nutrient transporters (MCT1 and GLUT1) in the developing eyes of mice

Kishimoto, Ayuko; Takahashi-Iwanaga, Hiromi; Watanabe, Masahiko M; Iwanaga, Toshihiko

Differential expression of endothelial nutrient transporters (MCT1 and GLUT1) in the developing eyes of mice

Kishimoto, Ayuko; Takahashi-Iwanaga, Hiromi; Watanabe, Masahiko M et al.

Experimental eye research, 2016, 153, 170-177

Permalink : https://hdl.handle.net/2115/67738

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/67738
タイトル	Differential expression of endothelial nutrient transporters (MCT1 and GLUT1) in the developing eyes of mice
著者	Kishimoto, Ayuko ; Takahashi-Iwanaga, Hiromi ; Watanabe, Masahiko M ; Iwanaga, Toshihiko
著者名 Kishimoto, Ayuko
e-Rad_Researcher 30193759 著者名 Takahashi-Iwanaga, Hiromi
著者名 Watanabe, Masahiko M
e-Rad_Researcher 10160128 著者名 Iwanaga, Toshihiko
言語	英語
キーワード	GLUT1
	MCT1
	Hyaloid artery
	Retina
	Angiogenesis
	Immunohistochemistry
発行日	2016-12
出版者	Elsevier
収録物名	Experimental eye research
巻(号)	153
ページ	170-177
抄録	The blood-brain barrier in the neonatal brain expresses the monocarboxylate transporter (MCT)-1 rather than the glucose transporter (GLUT)-1, due to the special energy supply during the suckling period. The hyaloid vascular system, consisting of the vasa hyaloidea propria and tunica vasculosa lentis, is a temporary vasculature present only during the early development of mammalian eyes and later regresses. Although the ocular vasculature manifests such a unique developmental process, no information is available concerning the expression of endothelial nutrient transporters in the developing eye. The present immunohistochemical study using whole mount preparations of murine eyes found that the hyaloid vascular system predominantly expressed GLUT1 in the endothelium, in contrast to the brain endothelium. Characteristically, the endothelium in peripheral regions of the neonatal hyaloid vessels displayed a mosaic pattern of MCT1-immunoreactive cells scattered within the GLUT1-expressing endothelium. The proper retinal vessels first developed by sprouting angiogenesis endowed with filopodia, which were absolutely free from the immunoreactivities of GLUT1 and MCT1. The remodeling retinal capillary networks and veins in the surface layer of the retina mainly expressed MCT1 until the weaning period. Immunostaining of MCT1 in the retina revealed fine radicular processes projecting from the endothelium, differing from the MCT1-immunonegative filopodia. These findings suggest that the expression of nutrient transporters in the ocular blood vessels is differentially regulated at a cellular level and that the neonatal eyes provide an interesting model for research on nutrient transporters in the endothelium.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0014-4835
関連情報	PMID 27793618
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.exer.2016.10.019