Infection of Epstein–Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines

Nanbo, Asuka; Katano, Harutaka; Kataoka, Michiyo; Hoshina, Shiho; Sekizuka, Tsuyoshi; Kuroda, Makoto; Ohba, Yusuke

Infection of Epstein–Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines

Nanbo, Asuka; Katano, Harutaka; Kataoka, Michiyo et al.

Cancers, 2018, 10(7), 237

Permalink : https://hdl.handle.net/2115/71077

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/71077
タイトル	Infection of Epstein–Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines
著者	Nanbo, Asuka ; Katano, Harutaka ; Kataoka, Michiyo ; Hoshina, Shiho ; Sekizuka, Tsuyoshi ; Kuroda, Makoto ; Ohba, Yusuke
e-Rad_Researcher 60359487 著者名 Nanbo, Asuka
著者名 Katano, Harutaka
著者名 Kataoka, Michiyo
著者名 Hoshina, Shiho
著者名 Sekizuka, Tsuyoshi
著者名 Kuroda, Makoto
e-Rad_Researcher 30333503 著者名 Ohba, Yusuke
言語	英語
キーワード	Epstein–Barr virus
	Burkitt’s lymphoma
	exosomes
	miRNA
	next-generation sequencing
発行日	2018-07
出版者	MDPI
収録物名	Cancers
巻(号)	10(7)
ページ	237
抄録	Infection of Epstein-Barr virus (EBV), a ubiquitous human gamma herpesvirus, is associated with various malignancies in B lymphocytes and epithelial cells. EBV encodes 49 microRNAs in two separated regions, termed the BART and BHRF1 loci. Although accumulating evidence demonstrates that EBV infection regulates the profile of microRNAs in the cells, little is known about the microRNAs in exosomes released from infected cells. Here, we characterized the expression profile of intracellular and exosomal microRNAs in EBV-negative, and two related EBV-infected Burkitt lymphoma cell lines having type I and type III latency by next-generation sequencing. We found that the biogenesis of exosomes is upregulated in type III latently infected cells compared with EBV-negative and type I latently infected cells. We also observed that viral and several specific host microRNAs were predominantly incorporated in the exosomes released from the cells in type III latency. We confirmed that multiple viral microRNAs were transferred to the epithelial cells cocultured with EBV-infected B cells. Our findings indicate that EBV infection, in particular in type III latency, modulates the biogenesis of exosomes and the profile of exosomal microRNAs, potentially contributing to phenotypic changes in cells receiving these exosomes.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	2072-6694
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/cancers10070237