Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains

Furuta, Yoshikazu; Harima, Hayato; Ito, Emiko; Maruyama, Fumito; Ohnishi, Naomi; Osaki, Ken; Ogawa, Hirohito; Squarre, David; Hang'ombe, Bernard Mudenda; Higashi, Hideaki

Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains

Furuta, Yoshikazu; Harima, Hayato; Ito, Emiko et al.

Msystems, 2018, 3(5), e00182-18

Permalink : https://hdl.handle.net/2115/72165

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/72165
タイトル	Loss of Bacitracin Resistance Due to a Large Genomic Deletion among Bacillus anthracis Strains
著者	Furuta, Yoshikazu ; Harima, Hayato ; Ito, Emiko ; Maruyama, Fumito ; Ohnishi, Naomi ; Osaki, Ken ; Ogawa, Hirohito ; Squarre, David ; Hang'ombe, Bernard Mudenda ; Higashi, Hideaki
著者名 Furuta, Yoshikazu
著者名 Harima, Hayato
著者名 Ito, Emiko
著者名 Maruyama, Fumito
著者名 Ohnishi, Naomi
著者名 Osaki, Ken
著者名 Ogawa, Hirohito
著者名 Squarre, David
著者名 Hang'ombe, Bernard Mudenda
e-Rad_Researcher 20311227 著者名 Higashi, Hideaki
言語	英語
キーワード	Bacillus anthracis
	Bacillus cereus group
	antibiotic resistance
	bacitracin
	genome analysis
	rRNA operon
	unequal crossing over
発行日	2018-09
出版者	American Society for Microbiology
収録物名	Msystems
巻(号)	3(5)
ページ	e00182-18
抄録	Bacillus anthracis is a Gram-positive endospore-forming bacterial species that causes anthrax in both humans and animals. In Zambia, anthrax cases are frequently reported in both livestock and wildlife, with occasional transmission to humans, causing serious public health problems in the country. To understand the genetic diversity of B. anthracis strains in Zambia, we sequenced and compared the genomic DNA of B. anthracis strains isolated across the country. Single nucleotide polymorphisms clustered these strains into three groups. Genome sequence comparisons revealed a large deletion in strains belonging to one of the groups, possibly due to unequal crossing over between a pair of rRNA operons. The deleted genomic region included genes conferring resistance to bacitracin, and the strains with the deletion were confirmed with loss of bacitracin resistance. Similar deletions between rRNA operons were also observed in a few B. anthracis strains phylogenetically distant from Zambian strains. The structure of bacitracin resistance genes flanked by rRNA operons was conserved only in members of the Bacillus cereus group. The diversity and genomic characteristics of B. anthracis strains determined in this study would help in the development of genetic markers and treatment of anthrax in Zambia. IMPORTANCE Anthrax is caused by Bacillus anthracis, an endospore-forming soil bacterium. The genetic diversity of B. anthracis is known to be low compared with that of Bacillus species. In this study, we performed whole-genome sequencing of Zambian isolates of B. anthracis to understand the genetic diversity between closely related strains. Comparison of genomic sequences revealed that closely related strains were separated into three groups based on single nucleotide polymorphisms distributed throughout the genome. A large genomic deletion was detected in the region containing a bacitracin resistance gene cluster flanked by rRNA operons, resulting in the loss of bacitracin resistance. The structure of the deleted region, which was also conserved among species of the Bacillus cereus group, has the potential for both deletion and amplification and thus might be enabling the species to flexibly control the level of bacitracin resistance for adaptive evolution.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
権利情報	Copyright © 2018 Furuta et al.
PISSN	2379-5077
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1128/mSystems.00182-18