Characterization of Campylobacter jejuni DNA gyrase as the target of quinolones

Changkwanyeun, Ruchirada; Usui, Masaru; Kongsoi, Siriporn; Yokoyama, Kazumasa; Kim, Hyun; Suthienkul, Orasa; Changkaew, Kanjana; Nakajima, Chie; Tamura, Yutaka; Suzuki, Yasuhiko

Characterization of Campylobacter jejuni DNA gyrase as the target of quinolones

Changkwanyeun, Ruchirada; Usui, Masaru; Kongsoi, Siriporn et al.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 2015, 21(8), 604-609

Permalink : https://hdl.handle.net/2115/76492

このアイテムのアクセス数:1,026件（2026-10-07 15:37 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Changkwanyeun_JIC_Main text_Rev_Accepted-Final	pdf	512 KB	778
MS_Changkwanyenun_JIC_Rev_Accepted_Fig S	pptx	253 KB	222

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/76492
タイトル	Characterization of Campylobacter jejuni DNA gyrase as the target of quinolones
著者	Changkwanyeun, Ruchirada ; Usui, Masaru ; Kongsoi, Siriporn ; Yokoyama, Kazumasa ; Kim, Hyun ; Suthienkul, Orasa ; Changkaew, Kanjana ; Nakajima, Chie ; Tamura, Yutaka ; Suzuki, Yasuhiko
著者名 Changkwanyeun, Ruchirada
著者名 Usui, Masaru
著者名 Kongsoi, Siriporn
著者名 Yokoyama, Kazumasa
e-Rad_Researcher 90648817 著者名 Kim, Hyun
著者名 Suthienkul, Orasa
著者名 Changkaew, Kanjana
e-Rad_Researcher 60435964 著者名 Nakajima, Chie
e-Rad_Researcher 50382487 著者名 Tamura, Yutaka
e-Rad_Researcher 90206540 著者名 Suzuki, Yasuhiko
言語	英語
キーワード	DNA gyrase
	Quinolones
	Campylobacter jejuni
発行日	2015-08
出版者	Elsevier
収録物名	Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
巻(号)	21(8)
ページ	604-609
抄録	Quinolones have long been used as the first-line treatment for Campylobacter infections. However, an increased resistance to quinolones has raised public health concerns. The development of new quinolone-based antibiotics with high activity is critical for effective, as DNA gyrase, the target of quinolones, is an essential enzyme for bacterial growth in several mechanisms. The evaluation of antibiotic activity against Campylobacter jejuni largely relies on drug susceptibility tests, which require at least 2 days to produce results. Thus, an in vitro method for studying the activity of quinolones against the C. jejuni DNA gyrase is preferred. To identify potent quinolones, we investigated the interaction of C. jejuni DNA gyrase with a number of quinolones using recombinant subunits. The combination of purified subunits exhibited DNA supercoiling activity in an ATP dependent manner. Drug concentrations that inhibit DNA supercoiling by 50% (IC50s) of 10 different quinolones were estimated to range from 0.4 (sitafloxacin) to >100 µg/mL (nalidixic acid). Sitafloxacin showed the highest inhibitory activity, and the analysis of the quinolone structure-activity relationship demonstrated that a fluorine atom at R-6 might play the important role in the inhibitory activity against C. jejuni gyrase. Measured quinolone IC50s correlated well with minimum inhibitory concentrations (R = 0.9943). These suggest that the in vitro supercoiling inhibition assay on purified recombinant C. jejuni DNA gyrase is a useful and predictive technique to monitor the antibacterial potency of quinolones. And furthermore, these data suggested that sitafloxacin might be a good candidate for clinical trials on campylobacteriosis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1341-321X
EISSN	1437-7780
NCID	AA11627318
関連情報	PMID 26096494
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jiac.2015.05.003