Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles

Furihata, Tomokazu; Miyaji, Hirofumi; Nishida, Erika; Kato, Akihito; Miyata, Saori; Shitomi, Kanako; Mayumi, Kayoko; Kanemoto, Yukimi; Sugaya, Tsutomu; Akasaka, Tsukasa

Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles

Furihata, Tomokazu; Miyaji, Hirofumi; Nishida, Erika et al.

Journal of biomedical materials research. Part B, Applied biomaterials, 2020, 108(7), 3033-3044

Permalink : https://hdl.handle.net/2115/82818

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アクセス権	open access
URI	https://hdl.handle.net/2115/82818
タイトル	Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles
著者	Furihata, Tomokazu ; Miyaji, Hirofumi ; Nishida, Erika ; Kato, Akihito ; Miyata, Saori ; Shitomi, Kanako ; Mayumi, Kayoko ; Kanemoto, Yukimi ; Sugaya, Tsutomu ; Akasaka, Tsukasa
著者名 Furihata, Tomokazu
e-Rad_Researcher 50372256 著者名 Miyaji, Hirofumi
e-Rad_Researcher 50779882 著者名 Nishida, Erika
e-Rad_Researcher 40507571 著者名 Kato, Akihito
著者名 Miyata, Saori
著者名 Shitomi, Kanako
著者名 Mayumi, Kayoko
著者名 Kanemoto, Yukimi
e-Rad_Researcher 10211301 著者名 Sugaya, Tsutomu
e-Rad_Researcher 00360917 著者名 Akasaka, Tsukasa
言語	英語
キーワード	bone filling material
	integrin β 1
	osteogenic differentiation
	rat skull
	recombinant peptide based on human collagen type I
発行日	2020-10
出版者	John Wiley & Sons
収録物名	Journal of biomedical materials research. Part B, Applied biomaterials
巻(号)	108(7)
ページ	3033-3044
抄録	Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl-glycyl-aspartic acid (RGD)-rich motif to enhance cell behavior and is anticipated as a xeno-free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta-tricalcium phosphate fine particles (RCP/β-TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β-TCP. Recombinant peptide was thermal crosslinked and freeze-dried to prepare RCP. An aqueous dispersion of β-TCP fine particles was added to RCP to obtain RCP/β-TCP. Subsequently, RCP/β-TCP were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β-TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β-TCP, β-TCP particles dose-dependently covered the surface of RCP. Cell culture tests showed that RCP/β-TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3-E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β-TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β-TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: [Furihata T, Miyaji H, Nishida E, et al. Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles. J Biomed Mater Res B Appl Biomater. 2020;10.1002/jbm.b.34632.], which has been published in final form at [https://doi.org/10.1002/jbm.b.34632]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	1552-4973
関連情報	PMID 32386261
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/jbm.b.34632