Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity

Watanabe, Mika; Kosumi, Hideyuki; Osada, Shin-Ichi; Takashima, Shota; Wang, Yunan; Nishie, Wataru; Oikawa, Tsukasa; Hirose, Tomonori; Shimizu, Hiroshi; Natsuga, Ken

Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity

Watanabe, Mika; Kosumi, Hideyuki; Osada, Shin-Ichi et al.

Experimental dermatology, 2021, 30(1), 62-67

Permalink : https://hdl.handle.net/2115/83791

このアイテムのアクセス数:604件（2026-02-07 16:21 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
Watanabe_et_al_HUSCUP	pdf	1.41 MB	568
Watanabe_et_al_HUSCUP_Suppl. Fig	pdf	473 KB	155

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/83791
タイトル	Type XVII collagen interacts with the aPKC-PAR complex and maintains epidermal cell polarity
著者	Watanabe, Mika ; Kosumi, Hideyuki ; Osada, Shin-Ichi ; Takashima, Shota ; Wang, Yunan ; Nishie, Wataru ; Oikawa, Tsukasa ; Hirose, Tomonori ; Shimizu, Hiroshi ; Natsuga, Ken
著者名 Watanabe, Mika
著者名 Kosumi, Hideyuki
e-Rad_Researcher 00244484 著者名 Osada, Shin-Ichi
著者名 Takashima, Shota
著者名 Wang, Yunan
e-Rad_Researcher 20443955 著者名 Nishie, Wataru
e-Rad_Researcher 20457055 著者名 Oikawa, Tsukasa
著者名 Hirose, Tomonori
e-Rad_Researcher 00146672 著者名 Shimizu, Hiroshi
e-Rad_Researcher 70645457 著者名 Natsuga, Ken
言語	英語
キーワード	atypical PKC
	cell polarity
	COL17
	epidermal stem cell
	PAR3
発行日	2021-01
出版者	John Wiley & Sons
収録物名	Experimental dermatology
巻(号)	30(1)
ページ	62-67
抄録	Type XVII collagen (COL17) is a transmembrane protein expressed in the basal epidermis. COL17 serves as a niche for epidermal stem cells, and although its reduction has been implicated in altering cell polarity and ageing of the epidermis, it is unknown how COL17 affects epidermal cell polarity. Here, we uncovered COL17 as a binding partner of the aPKC-PAR complex, which is a key regulating factor of cell polarity. Immunoprecipitation-immunoblot assay and protein-protein binding assay revealed that COL17 interacts with aPKC and PAR3. COL17 deficiency or epidermis-specific aPKC lambda deletion destabilized PAR3 distribution in the epidermis, while aPKC zeta knockout did not. Asymmetrical cell division was pronounced in COL17-null neonatal paw epidermis. These results show that COL17 is pivotal for maintaining epidermal cell polarity. Our study highlights the previously unrecognized role of COL17 in the basal keratinocytes.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: Watanabe, M, Kosumi, H, Osada, S‐I, et al. Type XVII collagen interacts with the aPKC‐PAR complex and maintains epidermal cell polarity. Exp Dermatol. 2021; 30: 62– 67., which has been published in final form at https://doi.org/10.1111/exd.14196. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	0906-6705
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1111/exd.14196