Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone

Nonoyama, Takayuki; Wang, Lei; Tsuda, Masumi; Suzuki, Yuki; Kiyama, Ryuji; Yasuda, Kazunori; Tanaka, Shinya; Nagata, Kousuke; Fujita, Ryosuke; Sakamoto, Naoya; Kawasaki, Noriyuki; Yurimoto, Hisayoshi; Gong, Jian Ping

Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone

Nonoyama, Takayuki; Wang, Lei; Tsuda, Masumi et al.

Advanced Healthcare Materials, 2021, 10(3), 2001731

Permalink : https://hdl.handle.net/2115/84005

このアイテムのアクセス数:542件（2026-02-14 16:41 集計）

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アクセス権	open access
URI	https://hdl.handle.net/2115/84005
タイトル	Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone
著者	Nonoyama, Takayuki ; Wang, Lei ; Tsuda, Masumi ; Suzuki, Yuki ; Kiyama, Ryuji ; Yasuda, Kazunori ; Tanaka, Shinya ; Nagata, Kousuke ; Fujita, Ryosuke ; Sakamoto, Naoya ; Kawasaki, Noriyuki ; Yurimoto, Hisayoshi ; Gong, Jian Ping
e-Rad_Researcher 50709251 著者名 Nonoyama, Takayuki
e-Rad_Researcher 70637975 著者名 Wang, Lei
e-Rad_Researcher 30431307 著者名 Tsuda, Masumi
著者名 Suzuki, Yuki
著者名 Kiyama, Ryuji
e-Rad_Researcher 20166507 著者名 Yasuda, Kazunori
e-Rad_Researcher 70261287 著者名 Tanaka, Shinya
著者名 Nagata, Kousuke
著者名 Fujita, Ryosuke
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
e-Rad_Researcher 50770278 著者名 Kawasaki, Noriyuki
e-Rad_Researcher 80191485 著者名 Yurimoto, Hisayoshi
e-Rad_Researcher 20250417 著者名 Gong, Jian Ping
言語	英語
キーワード	bone absorbable materials
	hydroxyapatite
	isotope microscopy
	osteogenesis
	tough hydrogels
発行日	2021-02-03
出版者	John Wiley & Sons
収録物名	Advanced Healthcare Materials
巻(号)	10(3)
ページ	2001731
抄録	Tough double network (DN) hydrogels are promising substitutes of soft supporting tissues such as cartilage and ligaments. For such applications, it is indispensable to robustly fix the hydrogels to bones with medically feasible methods. Recently, robustly bonding the DN hydrogels to defected bones of rabbits in vivo has been proved successful. The low crystalline hydroxyapatite (HAp) of calcium-phosphate-hydroxide salt coated on the surface layer of the DN hydrogels induced spontaneous osteogenesis penetrating into the semi-permeable hydrogels to form a gel/bone composite layer. In this work, the Ca-44 isotope-doped HAp/DN hydrogel is implanted in a defect of rabbit femoral bone and the dynamic osteogenesis process at the gel/bone interface is analyzed by tracing the calcium isotope ratio using isotope microscopy. The synthetic HAp hybridized on the surface layer of DN gel dissolves rapidly in the first two weeks by inflammation, and then the immature bone with a gradient structure starts to form in the gel region, reutilizing the dissolved Ca ions. These results reveal, for the first time, that synthetic HAp is reutilized for osteogenesis. These facts help to understand the lifetime of bone absorbable materials and to elucidate the mechanism of spontaneous, non-toxic, but strong fixation of hydrogels to bones.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is the peer reviewed version of the following article: Isotope Microscopic Observation of Osteogenesis Process Forming Robust Bonding of Double Network Hydrogel to Bone, which has been published in final form at https://doi.org/10.1002/adhm.202001731. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
PISSN	2192-2640
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/adhm.202001731