open access

https://hdl.handle.net/2115/90133

Growth of nitrite-oxidizing Nitrospira and ammonia-oxidizing Nitrosomonas in marine recirculating trickling biofilter reactors

英語

2022-08

John Wiley & Sons

Environmental microbiology

24(8)

3735-3750

Aerobic ammonia and nitrite oxidation reactions are fundamental biogeochemical reactions contributing to the global nitrogen cycle. Although aerobic nitrite oxidation yields 4.8-folds less Gibbs free energy ( increment G(r)) than aerobic ammonia oxidation in the NH4+-feeding marine recirculating trickling biofilter reactors operated in the present study, nitrite-oxidizing and not ammonia-oxidizing Nitrospira (sublineage IV) outnumbered ammonia-oxidizing Nitrosomonas (relative abundance; 53.8% and 7.59% respectively). CO2 assimilation efficiencies during ammonia or nitrite oxidation were 0.077 mu mol-(CO2)-C-14/mu mol-NH3 and 0.053-0.054 mu mol-(CO2)-C-14/mu mol-NO2- respectively, and the difference between ammonia and nitrite oxidation was much smaller than the difference of increment G(r). Free-energy efficiency of nitrite oxidation was higher than ammonia oxidation (31%-32% and 13% respectively), and high CO2 assimilation and free-energy efficiencies were a determinant for the dominance of Nitrospira over Nitrosomonas. Washout of Nitrospira and Nitrosomonas from the trickling biofilter reactors was also examined by quantitative PCR assay. Normalized copy numbers of Nitrosomonas amoA were 1.5- to 1.7-folds greater than Nitrospira nxrB and 16S rRNA gene in the reactor effluents. Nitrosomonas was more susceptible for washout than Nitrospira in the trickling biofilter reactors, which was another determinant for the dominance of Nitrospira in the trickling biofilter reactors.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Oshiki, M., Netsu, H., Kuroda, K., Narihiro, T., Fujii, N., Kindaichi, T., Suzuki, Y., Watari, T., Hatamoto, M., Yamaguchi, T., Araki, N. and Okabe, S. (2022), Growth of nitrite-oxidizing Nitrospira and ammonia-oxidizing Nitrosomonas in marine recirculating trickling biofilter reactors. Environ Microbiol, 24: 3735-3750, which has been published in final form at https://doi.org/10.1111/1462-2920.16085. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

1462-2912