Dopamine regulates astrocytic IL-6 expression and process formation via dopamine receptors and adrenoceptors

Morimoto, Kohei; Ouchi, Mai; Kitano, Taisuke; Eguchi, Ryota; Otsuguro, Ken-ichi

Dopamine regulates astrocytic IL-6 expression and process formation via dopamine receptors and adrenoceptors

Morimoto, Kohei; Ouchi, Mai; Kitano, Taisuke et al.

European journal of pharmacology, 2022, 928, 175110

Permalink : https://hdl.handle.net/2115/90384

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/90384
タイトル	Dopamine regulates astrocytic IL-6 expression and process formation via dopamine receptors and adrenoceptors
著者	Morimoto, Kohei ; Ouchi, Mai ; Kitano, Taisuke ; Eguchi, Ryota ; Otsuguro, Ken-ichi
著者名 Morimoto, Kohei
著者名 Ouchi, Mai
著者名 Kitano, Taisuke
著者名 Eguchi, Ryota
e-Rad_Researcher 40344494 著者名 Otsuguro, Ken-ichi
言語	英語
キーワード	Astrocyte
	Dopamine
	Adrenoceptor
	IL-6
	CREB
	Morphology
発行日	2022-08-05
出版者	Elsevier
収録物名	European journal of pharmacology
巻(号)	928
ページ	175110
抄録	Dopamine levels in the central nervous system change under pathological conditions such as Parkinson's disease, Huntington's disease, and addiction. Under those pathological conditions, astrocytes become reactive astrocytes characterized by morphological changes and the release of inflammatory cytokines involved in pathogenesis. However, it remains unclear whether dopamine regulates astrocytic morphology and functions. Elucidating these issues will help us to understand the pathogenesis of neurodegenerative diseases caused by abnormal dopamine signaling. In this study, we investigated the effects of dopamine on IL-6 expression and process formation in rat primary cultured astrocytes and acute hippocampal slices. Dopamine increased IL-6 expression in a concentration-dependent manner, and this was accompanied by CREB phosphorylation. The effects of a low dopamine concentration (1 mu M) were inhibited by a D1-like receptor antagonist, whereas the effects of a high dopamine concentration (100 mu M) were inhibited by a beta-antagonist and enhanced by a D2-like receptor antagonist. Furthermore, dopamine (100 mu M) promoted process formation, which was inhibited by a beta-antag-onist and enhanced by both an alpha-antagonist and a D2-like receptor antagonist. In acute hippocampal slices, both a D1-like receptor agonist and beta-agonist changed astrocytic morphology. Together, these results indicate that dopamine promotes IL-6 expression and process formation via D1-like receptors and beta-adrenoceptors. Further-more, bidirectional regulation exists; namely, the effects of D1-like receptors and beta-adrenoceptors were nega-tively regulated by D2-like receptors and alpha(2)-adrenoceptors.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© <2022>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0014-2999
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ejphar.2022.175110