PTBP2 binds to a testis-specific long noncoding RNA, Tesra, and activates transcription of the Prss42/Tessp-2 gene

Sato, Josei; Satoh, Yui; Yamamoto, Takehiro; Watanabe, Takehiro; Matsubara, Shin; Satake, Honoo; Kimura, Atsushi P.

PTBP2 binds to a testis-specific long noncoding RNA, Tesra, and activates transcription of the Prss42/Tessp-2 gene

Sato, Josei; Satoh, Yui; Yamamoto, Takehiro et al.

Gene, 2024, 893, 147907

Permalink : https://hdl.handle.net/2115/94013

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/94013
タイトル	PTBP2 binds to a testis-specific long noncoding RNA, Tesra, and activates transcription of the Prss42/Tessp-2 gene
著者	Sato, Josei ; Satoh, Yui ; Yamamoto, Takehiro ; Watanabe, Takehiro ; Matsubara, Shin ; Satake, Honoo ; Kimura, Atsushi P.
著者名 Sato, Josei
著者名 Satoh, Yui
e-Rad_Researcher 50383774 著者名 Yamamoto, Takehiro
著者名 Watanabe, Takehiro
e-Rad_Researcher 70710747 著者名 Matsubara, Shin
e-Rad_Researcher 20280688 著者名 Satake, Honoo
e-Rad_Researcher 90422005 著者名 Kimura, Atsushi P.
言語	英語
キーワード	Testis
	Long noncoding RNA
	RNA -binding protein
	Transcription
	Promoter
発行日	2024-01-30
出版者	Elsevier
収録物名	Gene
巻(号)	893
ページ	147907
抄録	Long noncoding RNAs (lncRNAs) have recently been proved to be functional in the testis. Tesra, a testis-specific lncRNA, was suggested to activate the transcription of Prss42/Tessp-2, a gene that is involved in meiotic progression, in mouse spermatocytes. To reveal the molecular mechanism underlying the activation, we searched for Tesra-binding proteins by a Ribotrap assay followed by LC-MS/MS analysis and identified polypyrimidine tract binding protein 2 (PTBP2) as a candidate. Analysis of public RNA-seq data and our qRT-PCR results indicated that Ptbp2 mRNA showed an expression pattern similar to the expression patterns of Tesra and Prss42/Tessp-2 during testis development. Moreover, PTBP2 was found to be associated with Tesra in testicular germ cells by RNA immunoprecipitation. To evaluate the effect of PTBP2 on the Prss42/Tessp-2 promoter, we established an in vitro reporter gene assay system in which Tesra expression could be induced by the Tet-on system and thereby Prss42/Tessp-2 promoter activity could be increased. In this system, the Prss42/Tessp-2 promoter activity was significantly decreased by the knockdown of PTBP2. These results suggest that PTBP2 contributes to Prss42/ Tessp-2 transcriptional activation by Tesra in spermatocytes. The finding provides a precious example of a molecular mechanism of testis lncRNA functioning in spermatogenesis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0378-1119
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.gene.2023.147907