open access

https://hdl.handle.net/2115/95931

In utero morphological and functional properties of bovine trophoblastic vesicles

英語

trophectoderm

2024-08-23

John Wiley & Sons

Molecular Reproduction and Development

91(8)

In many mammals, including ruminants, pregnancy requires pregnancy recognition signaling molecules secreted by the conceptus; however, the mechanism underlying pregnancy establishment in cattle remains unknown. Trophoblastic vesicles (TVs) are artificially produced from the extraembryonic tissues of the elongating conceptus and may be useful tools for understanding conception. This study investigated the morphological and functional properties of TVs in comparison to those of intact conceptuses. TVs were prepared from the extraembryonic tissues of conceptuses collected 14 days after artificial insemination (AI), cryopreserved immediately after dissection, and cultured after thawing for subsequent transplantation into the uterus. The transferred TVs were collected 7 days after transplantation and compared with extraembryonic tissue samples collected from conceptuses at 21 days post-AI. The recovered TVs were 40 times longer than those of their pre-transplant counterparts. Microscopic evaluation revealed that their membrane structures consisted of trophoblast and hypoblast layers. The expression patterns of the cell differentiation markers, CDX2, SOX2, and GATA6, and interferon tau (IFNT) protein expression levels in the TVs were similar to those in control extraembryonic tissue samples. These findings suggest that TVs are capable of morphological elongation and maintain IFNT production in a similar way as original trophoblasts. Trophoblastic vesicles (TVs) produced from extraembryonic tissues can be useful tools for understanding bovine conception. This study demonstrates that TVs are capable of morphological elongation and can maintain interferon production in a similar way as original trophoblasts. image

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Kagawa, S., Hayashi, Y., Bai, H., Takahashi, M., & Kawahara, M. (2024). In utero morphological and functional properties of bovine trophoblastic vesicles. Molecular Reproduction and Development, 91, e23767, which has been published in final form at https://doi.org/10.1002/mrd.23767. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

1040-452X