open access

https://hdl.handle.net/2115/97899

Chelatase activity of magnesium dechelatase associated with chlorophyll degradation

英語

substrate specificity

2025-06-17

John Wiley & Sons

FEBS Letters

599(15)

2210-2219

Metalated tetrapyrrole molecules play crucial roles in respiration, photosynthesis, and biocatalysis. In this study, the chelatase activity of the bacterial magnesium dechelatase homologous gene, which encodes an enzyme that extracts the central magnesium ion from chlorophyll, was demonstrated. The recombinant protein inserted metal ions, such as zinc, into methyl pyropheophorbide a, a synthetic derivative of chlorophyll a. Mutation analysis and structural modeling suggested H32, D34, and D62 as key residues involved in coordinating metal ions and facilitating proton extraction from methyl pyropheophorbide a. Chelatase activity was also found in the recombinant plant magnesium dechelatase protein. The bacterial gene complemented Escherichia coli ferrochelatase mutants. These findings provide novel insights into the mechanisms underlying chelation reactions.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Sato, S., Hirose, M., Tamiaki, H. and Ito, H. (2025), Chelatase activity of magnesium dechelatase associated with chlorophyll degradation. FEBS Lett, 599: 2210-2219., which has been published in final form at https://doi.org/10.1002/1873-3468.70094 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

0014-5793