Microbial degradation of the artificial polyhydroxyalkanoate poly(2-hydroxybutyrate-co-3-hydroxybutyrate) with random and block sequences using soil bacteria

Hachisuka, Shin-ichi; Iguchi, Ami; Kikukawa, Hiroshi; Tomita, Hiroya; Ooi, Toshihiko; Matsumoto, Ken'ichiro

Microbial degradation of the artificial polyhydroxyalkanoate poly(2-hydroxybutyrate-co-3-hydroxybutyrate) with random and block sequences using soil bacteria

Hachisuka, Shin-ichi; Iguchi, Ami; Kikukawa, Hiroshi et al.

Journal of applied microbiology, 2025, 136(6), lxaf123

Permalink : https://hdl.handle.net/2115/97913

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/97913
タイトル	Microbial degradation of the artificial polyhydroxyalkanoate poly(2-hydroxybutyrate-co-3-hydroxybutyrate) with random and block sequences using soil bacteria
著者	Hachisuka, Shin-ichi ; Iguchi, Ami ; Kikukawa, Hiroshi ; Tomita, Hiroya ; Ooi, Toshihiko ; Matsumoto, Ken'ichiro
e-Rad_Researcher 90835927 著者名 Hachisuka, Shin-ichi
著者名 Iguchi, Ami
e-Rad_Researcher 80758805 著者名 Kikukawa, Hiroshi
e-Rad_Researcher 00814229 著者名 Tomita, Hiroya
e-Rad_Researcher 40223713 著者名 Ooi, Toshihiko
e-Rad_Researcher 80360642 著者名 Matsumoto, Ken'ichiro
言語	英語
キーワード	polyhydroxyalkanoate
	poly(2-hydroxybutyrate-co-3-hydroxybutyrate)
	random copolymer
	block copolymer
	microbial degradation
発行日	2025-06
出版者	Oxford University Press
収録物名	Journal of applied microbiology
巻(号)	136(6)
ページ	lxaf123
抄録	Aims This study aimed to examine whether microorganisms capable of degrading the artificial polyhydroxyalkanoate (PHA) copolymer poly(2-hydroxybutyrate-co-3-hydroxybutyrate) [P(2HB-co-3HB)], with random and block sequences, are present in natural environments. Previously isolated PHA-degrading soil bacteria were tested using 2HB-rich copolymers, P(2HB-ran-3HB) and P(2HB)-b-P(3HB), as a major carbon source. The assimilation of 2HB and 3HB fractions in these copolymers and the effect of monomer sequence on degradability were investigated.Methods and results Acidovorax sp. YM1609, Cupriavidus sp. T1, and Variovorax spp. A25i and A60i2 were tested in clear zone analysis to determine the depolymerization of the polymer and GC analysis to assess the assimilation of the monomer component following depolymerization. Strains YM1609, A25i, and A60i2 depolymerized emulsified P(2HB-ran-3HB) and assimilated both components. For emulsified P(2HB)-b-P(3HB), all the strains depolymerized and assimilated the 3HB component but hardly did the 2HB component within 2 weeks.Conclusions This study revealed that both P(2HB-ran-3HB) and P(2HB)-b-P(3HB) can be degraded to some extent by soil bacteria. 2HB components were more susceptible to degradation in random sequence than in the block sequence; in contrast, the opposite trend was rather applied for 3HB components.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Applied Microbiology following peer review. The version of record Shin-ichi Hachisuka, Ami Iguchi, Hiroshi Kikukawa, Hiroya Tomita, Toshihiko Ooi, Ken'ichiro Matsumoto, Microbial degradation of the artificial polyhydroxyalkanoate poly(2-hydroxybutyrate-co-3-hydroxybutyrate) with random and block sequences using soil bacteria, Journal of Applied Microbiology, Volume 136, Issue 6, June 2025, lxaf123 is available online at: https://doi.org/10.1093/jambio/lxaf123.
PISSN	1364-5072
関連情報	PMID 40392676
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/jambio/lxaf123