Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials

Tsuji, Erika; Sumi, Kanako; Tsuzuki, Natsuko; Kondoh, Daisuke; Tsujio, Masashi; Hosotani, Marina; Namba, Takashi; Wakitani, Shoichi; Ichii, Osamu; Nakanishi, Ko; Nakamura, Teppei

Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials

Tsuji, Erika; Sumi, Kanako; Tsuzuki, Natsuko et al.

Microscopy and microanalysis, 2025, 31(4)

Permalink : https://hdl.handle.net/2115/97956

このアイテムのアクセス数:78件（2026-08-24 16:08 集計）

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/97956
タイトル	Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials
著者	Tsuji, Erika ; Sumi, Kanako ; Tsuzuki, Natsuko ; Kondoh, Daisuke ; Tsujio, Masashi ; Hosotani, Marina ; Namba, Takashi ; Wakitani, Shoichi ; Ichii, Osamu ; Nakanishi, Ko ; Nakamura, Teppei
著者名 Tsuji, Erika
著者名 Sumi, Kanako
著者名 Tsuzuki, Natsuko
著者名 Kondoh, Daisuke
著者名 Tsujio, Masashi
著者名 Hosotani, Marina
著者名 Namba, Takashi
著者名 Wakitani, Shoichi
著者名 Ichii, Osamu
著者名 Nakanishi, Ko
e-Rad_Researcher 80786773 著者名 Nakamura, Teppei
言語	英語
キーワード	bone biomaterials
	bone histology
	comparative biology
	histological staining
	inter-laboratory validation
	intra-laboratory validation
	JFRL staining
	osteochondral components
	reproducibility
	robustness
発行日	2025-07-15
出版者	Oxford University Press
収録物名	Microscopy and microanalysis
巻(号)	31(4)
抄録	Histological staining is essential for understanding bone structure and pathology; however, variations in decalcification agents can compromise reproducibility. We have previously developed a novel osteochondral staining method, Join of the Five dyes Revealing coLlagenous tissue (JFRL) staining, that is independent of the decalcification method. To promote its widespread adoption, this study confirms the robustness of JFRL staining through intra- and inter-laboratory validation. JFRL staining demonstrated consistent patterns across different manufacturers and facilities, with proper dehydration steps being crucial for optimal results. We applied JFRL staining to diverse vertebrate species prepared under various fixation and decalcification conditions to effectively visualize species-specific bone structures, including distinct osteoid and mineralized bone features from fish to large mammals. Furthermore, JFRL staining proved useful in evaluating bone biomaterials within defect models and clearly depicts the complex architecture of bone-healing processes and material integration. The staining qualitatively distinguished osteoid, mineralized bone, hyaline cartilage, and bone cells of different colors across all applications. These findings establish JFRL staining as a robust and versatile method for bone histology. Future studies focusing on quantitative assessment and pathological applications will prove that JFRL staining presents a reliable tool for both basic research and clinical diagnostics of bone disorders.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in Microscopy and Microanalysis following peer review. The version of record Erika Tsuji, Kanako Sumi, Natsuko Tsuzuki, Daisuke Kondoh, Masashi Tsujio, Marina Hosotani, Takashi Namba, Shoichi Wakitani, Osamu Ichii, Ko Nakanishi, Teppei Nakamura, Multi-laboratory Validation of Join of the Five Dyes Revealing Collagenous Tissue (JFRL) Staining for Bone Histology Across Animal Species and Bone Implant Materials, Microscopy and Microanalysis, Volume 31, Issue 4, August 2025, ozaf058 is available online at: https://doi.org/10.1093/mam/ozaf058
PISSN	1431-9276
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/mam/ozaf058