Japanese Journal of Veterinary Research;Volume 3, Number 2

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STUDIES ON A PYROGENIC SUBSTANCE IN BLOOD PLASMA OF VIROSIS

HONMA, Keizo;NAKANO, Osamu;MAKINO, Mikio;HONMA, Shokichi

Permalink : http://hdl.handle.net/2115/1669
JaLCDOI : 10.14943/jjvr.3.2.82

Abstract

In plasma in equine infectious anemia there was found a pyrogenic substance which caused a febrile response when injected intravenously in rabbits. This substance appeared in plasma of the diseased animals which suffered from fever fit, only when the fever fit was over, and it disappeared in about a week. The substance was insoluble in certain organic solvents such as ether-ethanol, acetone and chloroform. By salting-out method it was found in euglobulin fraction, and by method 9 of ONCLEY in III-O fraction. It has been proven undialysible with cellophane, and was not destroyed by boiling for 30 minutes at pH 6.6 It was active in producing fever over a pH range of 4 to 10.5 and maintained potency at room temperature for 72 hours, or at 0℃ for as long as 3 months. By COHN'S method for fractionation it was treated at -6℃ for about 3 days ; its potency remained. To apply the X-ray of 1200 Rontgen units resulted in no change of its activity. The electrophoretic pattern of pyrogenic III-O fraction revealed that the greater part of this substance was in β-globulin. Rabbits given daily intravenous injection of this substance for one week showed no sign of tolerance.

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