Japanese Journal of Veterinary Research;Volume 3, Number 3

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BIOCHEMICAL STUDIES ON THE INCREASE AND FORMATION OF ANTIBODIES WITH CHYMOPAPAIN IN VITRO : IV. STUDIES ON THE FORMATION OF DIPHTHERIAL ANTITOXIN FROM PRELIMINARILY PEPSIN-OR TRYPSIN-DIGESTED NORMAL EQUINE SERUM GLOBULINS

ITO, Tokiya

Permalink : http://hdl.handle.net/2115/1677
JaLCDOI : 10.14943/jjvr.3.3.147

Abstract

The previous papers have reported studies on the increase of diphtherial antitoxic titers in vitro from the preliminarily pepsin- or trypsin-digested, acid- or alkali-heated, or with urea or with sodium salicylate pre-denaturated diphtherial antitoxic equine serum globulins, these globulins were obtained from immunized horses during the course of progressive diphtherial immunization, incubating with diphtherial toxin (antigen) and oxidized chymopapain (catalyzer) at pH 7.3,Eh 450〜500 mV and 40℃. The process and mechanism of antibody formation have invited numerous studies ever since effective techniques for immunization have been found. Although the studies of the investigators to produce antibodies in vitro have brought remarkable advance in this field, lack of enzymechemical studies in the problem led the present authors to undertake some enzymic works on the formation and production of antibodies in vitro. The present paper deals with negative results of studies on the formation of diphtherial antitoxin incubating preliminarily pepsin- or trypsin-digested normal equine serum globulins with toxic solutions and oxidized chymopapain.

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