Japanese Journal of Veterinary Research;Volume 13, Number 3

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STUDIES ON HEMOLYTIC ESCHERICHIA COLI OF O-139 A CERTAIN FACTOR ESSENTIAL FOR HEMOLYSIN PRODUCTION

INUKAI, Yoshikazu;KODAMA, Hirohide

Permalink : http://hdl.handle.net/2115/1826
JaLCDOI : 10.14943/jjvr.13.3.87

Abstract

1) Considerable amounts of filtrable (soluble) hemolysin can be obtained by a simple hemolysin-producing system, namely the cell suspension system. 2) The development of filtrable hemolysin in the cell suspension system depends neither upon autolysis nor upon physical extraction of preformed hemolysin but upon hemolysin synthesis de novo. 3) The appearance of filtrable hemolysin in the cell suspension system is required a special factor (the I.H.P.E. factor) essentially, in addition to some nutrient factors. 4) The I.H.P.E. factor is contained in some meat infusion broth, cow's milk and egg white, but not in ordinary peptones and yeast extracts etc., and is an undialyzable substance, presumably a protein with high molecular weight. 5) It seems that the I.H.P.E. factor may not be related to the process of hemolysin synthesis, but to the appearance of hemolysin in the environment medium. 6) The most favorable conditions for hemolysin production in the cell suspension system are approximately pH 7,temperature 37℃, incubation period 30 min, respectively.

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