Japanese Journal of Veterinary Research;Volume 15, Number 3

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THREE TYPES OF CORYNEBACTERIUM RENALE CLASSIFIED BY PRECIPITIN REACTIONS IN GELS

YANAGAWA, Ryo;BASRI, Hasan;OTSUKI, Koichi

Permalink : http://hdl.handle.net/2115/1883
JaLCDOI : 10.14943/jjvr.15.3.111

Abstract

Serologically antigenic fractions were extracted from the cells of Corynebacterium renale by various agents. Treatment with sodium deoxycholate was found most satisfactory because it produced fractions containing antigens which formed several lines including all those found in HCl, ethanol, n-butanol, phenol and ether extracts. The antigens possessed a particle size of more than 10 mμ after being solubilized by deoxycholate and dialyzed. Antigenicity was similar between the antigens produced from the whole cell and the isolated cell wall fraction. Ultrathin sections of the cells treated with deoxycholate were shown. Typing of 78 strains of Corynebacterium renale by precipitin reaction in gels was performed using the antigen extracted with deoxycholate. The authors came to the conclusion that they were divided into 3 types. Some biochemical and cultural behavior characteristics were found helpful in explaining the differences between the types. Type I, predominant in number, and type II included strains originating from both normal urine and the material of pyelonephritis. Type III, least in number, was composed of all the isolates from the affected cattle.

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