Japanese Journal of Veterinary Research;Volume 15, Number 4

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STUDIES ON ECHINOCOCCOSIS XX : PRELIMINARY OBSERVATIONS OF THE IN VIVO CULTIVATION OF LARVAL TISSUE OF ECHINOCOCCUS MULTILOCULARIS IN CULTURE-CHAMBER OF POROUS MEMBRANE

SAKAMOTO, Tsukasa;KOTANI, Tadao

Permalink : http://hdl.handle.net/2115/1888
JaLCDOI : 10.14943/jjvr.15.4.165

Abstract

Echinococcal tissue consisted essentially of 300 to 500 scolices, a few germinal cells and small pieces of brood capsule, was introduced into the culture-chamber made of porous membrane of 0.45±0.02μ in pore-size, composed of cellulose ester, and silicon gum tube, 3.5^2π×7mm^3 in capacity. Three or 4 culture-chambers containing echinococcal tissue were inserted into abdominal cavity of a cotton rat, and the development of echinococcal tissue was observed morphologically in the course of time. The results obtained are as follows. On the 3rd day, vesicles which were lined by typical germinal cells and showed initial brood capsule formation filled up the space of the culture-chamber. On the 80th day, many mature brood capsules with scolices fully developed, daughter cysts and numerous calcareous corpuscles were present in the vesicles. The culture-chamber was full of vesicles with a remarkable number of brood capsules containing many scolices after 6 months. No multilocular vesiculation by exogenous budding was recognized.

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