Japanese Journal of Veterinary Research;Volume 15, Number 4

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MULTIPLICATION OF INFECTIOUS CANINE HEPATITIS VIRUS IN HELA CELLS

KINJO, Toshio;YANAGAWA, Ryo

Permalink : http://hdl.handle.net/2115/1885
JaLCDOI : 10.14943/jjvr.15.4.141

Abstract

HeLa cells were capable of supporting partial multiplication of infectious canine hepatitis (ICH) virus, the strain Woc-4. Infective virus grown in HeLa cells was low in titer despite the fact that the virus antigens were detected serologically and by the fluorescent antibody technique. Also deoxyribonucleic acid synthesis was demonstrable by acridine orange staining nearly as much as those detected in dog kidney cells. Thus it was considered that HeLa cells were able to support the synthesis of viral components but could not properly support the stage of assembly. By electron microscopic observation, the virus materials grown in HeLa cells were found to contain many empty, incomplete virus particles.

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