Japanese Journal of Veterinary Research;Volume 20, Number 4

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CLASSIFICATION OF CHICKEN COAGULASE-POSITIVE STAPHYLOCOCCI INTO FOUR BIOLOGICAL TYPES AND RELATION OF THE TYPES TO ADDITIONAL CHARACTERISTICS INCLUDING COAGULASE-ANTIGENIC TYPE

SATO, Gihei;MIURA, Shiro;TERAKADO, Nobuyuki

Permalink : http://hdl.handle.net/2115/2009
JaLCDOI : 10.14943/jjvr.20.4.91

Abstract

A total of 1,021 (98.4%) of 1,038 chicken coagulase-positive staphylococcal strains obtained from 27 farms were classified into 4 biological types (types 1~4) on the basis of properties such as pigmentation, hemolysis on sheep blood agar plate, Voges-Proskauer reaction and fermentation of lactose, galactose or mannitol. Only type 2 strains gave no pigment and were non-hemolytic. Voges-Proskauer reaction was negative, except for type 3,lactose- and galactose-fermentation was negative only in type 1,and mannitol fermentation was positive in all but type 2. DNase production was observed in all the biological types and egg-yolk factor in types 3 and 4. Caseinase was produced in about 80% of strains of each type. Fibrinolysin production was of low frequency, with the exception of type 2 strains. Only type 3 strains were phage-typed (about 38% of the type). Types 1 and 4 strains were classified into coagulase-antigenic type V, and type 3 strains into VII, VI and II of the coagulase type. Type 2 strains could not be coagulase-typed because of insufficient coagulase production, and indicated low virulence for mice and chickens.

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